2axc: Difference between revisions
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[[Image:2axc.gif|left|200px]] | [[Image:2axc.gif|left|200px]] | ||
'''Crystal structure of ColE7 translocation domain''' | {{Structure | ||
|PDB= 2axc |SIZE=350|CAPTION= <scene name='initialview01'>2axc</scene>, resolution 1.70Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= | |||
|GENE= colE7 (AMINO ACIDS 60-316) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Crystal structure of ColE7 translocation domain''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2AXC is a [ | 2AXC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AXC OCA]. | ||
==Reference== | ==Reference== | ||
High-resolution crystal structure of a truncated ColE7 translocation domain: implications for colicin transport across membranes., Cheng YS, Shi Z, Doudeva LG, Yang WZ, Chak KF, Yuan HS, J Mol Biol. 2006 Feb 10;356(1):22-31. Epub 2005 Dec 5. PMID:[http:// | High-resolution crystal structure of a truncated ColE7 translocation domain: implications for colicin transport across membranes., Cheng YS, Shi Z, Doudeva LG, Yang WZ, Chak KF, Yuan HS, J Mol Biol. 2006 Feb 10;356(1):22-31. Epub 2005 Dec 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16360169 16360169] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: translocation domain]] | [[Category: translocation domain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:55:03 2008'' | ||
Revision as of 16:55, 20 March 2008
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| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | colE7 (AMINO ACIDS 60-316) (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of ColE7 translocation domain
OverviewOverview
ColE7 is a nuclease-type colicin released from Escherichia coli to kill sensitive bacterial cells by degrading the nucleic acid molecules in their cytoplasm. ColE7 is classified as one of the group A colicins, since the N-terminal translocation domain (T-domain) of the nuclease-type colicins interact with specific membrane-bound or periplasmic Tol proteins during protein import. Here, we show that if the N-terminal tail of ColE7 is deleted, ColE7 (residues 63-576) loses its bactericidal activity against E.coli. Moreover, TolB protein interacts directly with the T-domain of ColE7 (residues 1-316), but not with the N-terminal deleted T-domain (residues 60-316), as detected by co-immunoprecipitation experiments, confirming that the N-terminal tail is required for ColE7 interactions with TolB. The crystal structure of the N-terminal tail deleted ColE7 T-domain was determined by the multi-wavelength anomalous dispersion method at a resolution of 1.7 angstroms. The structure of the ColE7 T-domain superimposes well with the T-domain of ColE3 and TR-domain of ColB, a group A Tol-dependent colicin and a group B TonB-dependent colicin, respectively. The structural resemblance of group A and B colicins implies that the two groups of colicins may share a mechanistic connection during cellular import.
About this StructureAbout this Structure
2AXC is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
High-resolution crystal structure of a truncated ColE7 translocation domain: implications for colicin transport across membranes., Cheng YS, Shi Z, Doudeva LG, Yang WZ, Chak KF, Yuan HS, J Mol Biol. 2006 Feb 10;356(1):22-31. Epub 2005 Dec 5. PMID:16360169
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