4aab: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[ | ==Crystal structure of the mutant D75N I-CreI in complex with its wild- type target (The four central bases, 2NN region, are composed by GTAC from 5' to 3')== | ||
<StructureSection load='4aab' size='340' side='right' caption='[[4aab]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4aab]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AAB FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1g9z|1g9z]], [[1bp7|1bp7]], [[1af5|1af5]], [[2vbl|2vbl]], [[2vbj|2vbj]], [[2vbo|2vbo]], [[1mow|1mow]], [[1g9y|1g9y]], [[2vbn|2vbn]], [[1n3f|1n3f]], [[1t9i|1t9i]], [[1u0d|1u0d]], [[1t9j|1t9j]], [[1n3e|1n3e]], [[1u0c|1u0c]], [[4aaf|4aaf]], [[4aad|4aad]], [[4aae|4aae]], [[4aag|4aag]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aab FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aab OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4aab RCSB], [http://www.ebi.ac.uk/pdbsum/4aab PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Homing endonucleases represent protein scaffolds that provide powerful tools for genome manipulation, as these enzymes possess a very low frequency of DNA cleavage in eukaryotic genomes due to their high specificity. The basis of protein-DNA recognition must be understood to generate tailored enzymes that target the DNA at sites of interest. Protein-DNA interaction engineering of homing endonucleases has demonstrated the potential of these approaches to create new specific instruments to target genes for inactivation or repair. Protein-DNA interface studies have been focused mostly on specific contacts between amino acid side chains and bases to redesign the binding interface. However, it has been shown that 4 bp in the central DNA sequence of the 22-bp substrate of a homing endonuclease (I-CreI), which do not show specific protein-DNA interactions, is not devoid of content information. Here, we analyze the mechanism of target discrimination in this substrate region by the I-CreI protein, determining how it can occur independently of the specific protein-DNA interactions. Our data suggest the important role of indirect readout in this substrate region, opening the possibility for a fully rational search of new target sequences, thus improving the development of redesigned enzymes for therapeutic and biotechnological applications. | |||
Non-specific protein-DNA interactions control I-CreI target binding and cleavage.,Molina R, Redondo P, Stella S, Marenchino M, D'Abramo M, Gervasio FL, Charles Epinat J, Valton J, Grizot S, Duchateau P, Prieto J, Montoya G Nucleic Acids Res. 2012 Apr 11. PMID:22495931<ref>PMID:22495931</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Endonuclease|Endonuclease]] | *[[Endonuclease|Endonuclease]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Chlamydomonas reinhardtii]] | [[Category: Chlamydomonas reinhardtii]] | ||
[[Category: Abramo, M D | [[Category: Abramo, M D]] | ||
[[Category: Duchateau, P | [[Category: Duchateau, P]] | ||
[[Category: Epinat, J C | [[Category: Epinat, J C]] | ||
[[Category: Gervasio, F L | [[Category: Gervasio, F L]] | ||
[[Category: Grizot, S | [[Category: Grizot, S]] | ||
[[Category: Marenchino, M | [[Category: Marenchino, M]] | ||
[[Category: Molina, R | [[Category: Molina, R]] | ||
[[Category: Montoya, G | [[Category: Montoya, G]] | ||
[[Category: Prieto, J | [[Category: Prieto, J]] | ||
[[Category: Redondo, P | [[Category: Redondo, P]] | ||
[[Category: Stella, S | [[Category: Stella, S]] | ||
[[Category: Valton, J | [[Category: Valton, J]] | ||
[[Category: Gene targeting]] | [[Category: Gene targeting]] | ||
[[Category: Homing endonuclease]] | [[Category: Homing endonuclease]] | ||
[[Category: Hydrolase-dna complex]] | [[Category: Hydrolase-dna complex]] | ||
[[Category: Protein-dna interaction]] | [[Category: Protein-dna interaction]] | ||