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[[ | ==A. fulgidus IscS-IscU complex structure== | ||
<StructureSection load='4eb5' size='340' side='right' caption='[[4eb5]], [[Resolution|resolution]] 2.53Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4eb5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EB5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EB5 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">iscS2, AF_0564 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 Archaeoglobus fulgidus DSM 4304]), AF_0185, AF_0565 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 Archaeoglobus fulgidus DSM 4304])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine_desulfurase Cysteine desulfurase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.7 2.8.1.7] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eb5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eb5 RCSB], [http://www.ebi.ac.uk/pdbsum/4eb5 PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/ISCS2_ARCFU ISCS2_ARCFU]] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity). [[http://www.uniprot.org/uniprot/O34393_ARCFU O34393_ARCFU]] A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The center of attention: IscS cysteine desulfurases and IscU scaffolds are involved in biological iron-sulfur cluster assembly. The X-ray structure of an anaerobically produced, mutated (Fe(2) S(2) -(IscS-IscU(D35A) ))(2) complex reveals a cluster coordinated by three IscU cysteines and the IscS active cysteine. In air-exposed crystals the cluster is oxidized to an Fe(2) S-S center; D35 is essential for complex dissociation. | |||
(IscS-IscU)(2) Complex Structures Provide Insights into Fe(2) S(2) Biogenesis and Transfer.,Marinoni EN, de Oliveira JS, Nicolet Y, Raulfs EC, Amara P, Dean DR, Fontecilla-Camps JC Angew Chem Int Ed Engl. 2012 Apr 18. doi: 10.1002/anie.201201708. PMID:22511353<ref>PMID:22511353</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Archaeoglobus fulgidus dsm 4304]] | [[Category: Archaeoglobus fulgidus dsm 4304]] | ||
[[Category: Cysteine desulfurase]] | [[Category: Cysteine desulfurase]] | ||
[[Category: Amara, P | [[Category: Amara, P]] | ||
[[Category: Dean, D R | [[Category: Dean, D R]] | ||
[[Category: Fontecilla-Camps, J C | [[Category: Fontecilla-Camps, J C]] | ||
[[Category: Marinoni, E N | [[Category: Marinoni, E N]] | ||
[[Category: Nicolet, Y | [[Category: Nicolet, Y]] | ||
[[Category: Oliveira, J S.de | [[Category: Oliveira, J S.de]] | ||
[[Category: Raulfs, E C | [[Category: Raulfs, E C]] | ||
[[Category: Scaffold]] | [[Category: Scaffold]] | ||
[[Category: Transferase-metal binding protein complex]] | [[Category: Transferase-metal binding protein complex]] | ||
Revision as of 03:42, 25 December 2014
A. fulgidus IscS-IscU complex structureA. fulgidus IscS-IscU complex structure
Structural highlights
Function[ISCS2_ARCFU] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity). [O34393_ARCFU] A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity). Publication Abstract from PubMedThe center of attention: IscS cysteine desulfurases and IscU scaffolds are involved in biological iron-sulfur cluster assembly. The X-ray structure of an anaerobically produced, mutated (Fe(2) S(2) -(IscS-IscU(D35A) ))(2) complex reveals a cluster coordinated by three IscU cysteines and the IscS active cysteine. In air-exposed crystals the cluster is oxidized to an Fe(2) S-S center; D35 is essential for complex dissociation. (IscS-IscU)(2) Complex Structures Provide Insights into Fe(2) S(2) Biogenesis and Transfer.,Marinoni EN, de Oliveira JS, Nicolet Y, Raulfs EC, Amara P, Dean DR, Fontecilla-Camps JC Angew Chem Int Ed Engl. 2012 Apr 18. doi: 10.1002/anie.201201708. PMID:22511353[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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