2aj0: Difference between revisions
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[[Image:2aj0.gif|left|200px]] | [[Image:2aj0.gif|left|200px]] | ||
'''Solution structure of apoCadA''' | {{Structure | ||
|PDB= 2aj0 |SIZE=350|CAPTION= <scene name='initialview01'>2aj0</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Cadmium-exporting_ATPase Cadmium-exporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.3 3.6.3.3] | |||
|GENE= cadA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1639 Listeria monocytogenes]) | |||
}} | |||
'''Solution structure of apoCadA''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2AJ0 is a [ | 2AJ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJ0 OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis for metal binding specificity: the N-terminal cadmium binding domain of the P1-type ATPase CadA., Banci L, Bertini I, Ciofi-Baffoni S, Su XC, Miras R, Bal N, Mintz E, Catty P, Shokes JE, Scott RA, J Mol Biol. 2006 Feb 24;356(3):638-50. Epub 2005 Dec 5. PMID:[http:// | Structural basis for metal binding specificity: the N-terminal cadmium binding domain of the P1-type ATPase CadA., Banci L, Bertini I, Ciofi-Baffoni S, Su XC, Miras R, Bal N, Mintz E, Catty P, Shokes JE, Scott RA, J Mol Biol. 2006 Feb 24;356(3):638-50. Epub 2005 Dec 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16388822 16388822] | ||
[[Category: Cadmium-exporting ATPase]] | [[Category: Cadmium-exporting ATPase]] | ||
[[Category: Listeria monocytogenes]] | [[Category: Listeria monocytogenes]] | ||
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[[Category: metal binding protein]] | [[Category: metal binding protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:50:15 2008'' | ||
Revision as of 16:50, 20 March 2008
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| Gene: | cadA (Listeria monocytogenes) | ||||||
| Activity: | Cadmium-exporting ATPase, with EC number 3.6.3.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of apoCadA
OverviewOverview
In bacteria, P1-type ATPases are responsible for resistance to di- and monovalent toxic heavy metals by taking them out of the cell. These ATPases have a cytoplasmic N terminus comprising metal binding domains defined by a betaalphabetabetaalphabeta fold and a CXXC metal binding motif. To check how the structural properties of the metal binding site in the N terminus can influence the metal specificity of the ATPase, the first structure of a Cd(II)-ATPase N terminus was determined by NMR and its coordination sphere was investigated by X-ray absorption spectroscopy. A novel metal binding environment was found, comprising the two conserved Cys residues of the metal binding motif and a Glu in loop 5. A bioinformatic search identifies an ensemble of highly homologous sequences presumably with the same function. Another group of highly homologous sequences is found which can be referred to as zinc-detoxifying P1-type ATPases with the metal binding pattern DCXXC in the N terminus. Because no carboxylate groups participate in Cu(I) or Ag(I) binding sites, we suggest that the acidic residue plays a key role in the coordination properties of divalent cations, hence conferring a function to the N terminus in the metal specificity of the ATPase.
About this StructureAbout this Structure
2AJ0 is a Single protein structure of sequence from Listeria monocytogenes. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for metal binding specificity: the N-terminal cadmium binding domain of the P1-type ATPase CadA., Banci L, Bertini I, Ciofi-Baffoni S, Su XC, Miras R, Bal N, Mintz E, Catty P, Shokes JE, Scott RA, J Mol Biol. 2006 Feb 24;356(3):638-50. Epub 2005 Dec 5. PMID:16388822
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