2j57: Difference between revisions

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==About this Structure==
==About this Structure==
2J57 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans]] with CU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Amine_dehydrogenase Amine dehydrogenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.99.3 1.4.99.3]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J57 OCA]].  
2J57 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Amine_dehydrogenase Amine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.99.3 1.4.99.3] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J57 OCA].  


==Reference==
==Reference==
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[[Category: transport]]
[[Category: transport]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 17:24:27 2007''
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 13:20:01 2007''

Revision as of 14:14, 5 November 2007

File:2j57.gif


2j57, resolution 2.25Å

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X-RAY REDUCED PARACCOCUS DENITRIFICANS METHYLAMINE DEHYDROGENASE N-QUINOL IN COMPLEX WITH AMICYANIN.

OverviewOverview

X-ray exposure during crystallographic data collection can result in, unintended redox changes in proteins containing functionally important, redox centers. In order to directly monitor X-ray-derived redox changes in, trapped oxidative half-reaction intermediates of Paracoccus denitrificans, methylamine dehydrogenase, a commercially available single-crystal UV/Vis, microspectrophotometer was installed on-line at the BioCARS beamline, 14-BM-C at the Advanced Photon Source, Argonne, USA. Monitoring the redox, state of the intermediates during X-ray exposure permitted the creation of, a general multi-crystal data collection strategy to generate true, structures of each redox intermediate.

About this StructureAbout this Structure

2J57 is a Protein complex structure of sequences from Paracoccus denitrificans with CU as ligand. Active as Amine dehydrogenase, with EC number 1.4.99.3 Structure known Active Site: AC1. Full crystallographic information is available from OCA.

ReferenceReference

Tracking X-ray-derived redox changes in crystals of a methylamine dehydrogenase/amicyanin complex using single-crystal UV/Vis microspectrophotometry., Pearson AR, Pahl R, Kovaleva EG, Davidson VL, Wilmot CM, J Synchrotron Radiat. 2007 Jan;14(Pt 1):92-8. Epub 2006 Dec 15. PMID:17211075

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