2a5f: Difference between revisions

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Revision as of 16:45, 20 March 2008

File:2a5f.gif

, resolution 2.02Å

Ligands:

, , , and

Gene:

ARF6 (Homo sapiens), ctxA, toxA (Vibrio cholerae)

Activity:

NAD(+)--diphthamide ADP-ribosyltransferase, with EC number 2.4.2.36

Coordinates:

save as pdb, mmCIF, xml

Cholera toxin A1 subunit bound to its substrate, NAD+, and its human protein activator, ARF6

OverviewOverview

The Vibrio cholerae bacterium causes devastating diarrhea when it infects the human intestine. The key event is adenosine diphosphate (ADP)-ribosylation of the human signaling protein GSalpha, catalyzed by the cholera toxin A1 subunit (CTA1). This reaction is allosterically activated by human ADP-ribosylation factors (ARFs), a family of essential and ubiquitous G proteins. Crystal structures of a CTA1:ARF6-GTP (guanosine triphosphate) complex reveal that binding of the human activator elicits dramatic changes in CTA1 loop regions that allow nicotinamide adenine dinucleotide (NAD+) to bind to the active site. The extensive toxin:ARF-GTP interface surface mimics ARF-GTP recognition of normal cellular protein partners, which suggests that the toxin has evolved to exploit promiscuous binding properties of ARFs.

About this StructureAbout this Structure

2A5F is a Protein complex structure of sequences from Homo sapiens and Vibrio cholerae. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:16099990

Page seeded by OCA on Thu Mar 20 15:45:35 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2a5f.gif|left|200px]]<br /><applet load="2a5f" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2a5f.gif|left|200px]]
-caption="2a5f, resolution 2.02&Aring;" />
-'''Cholera toxin A1 subunit bound to its substrate, NAD+, and its human protein activator, ARF6'''<br />
+ {{Structure
+|PDB= 2a5f |SIZE=350|CAPTION= <scene name='initialview01'>2a5f</scene>, resolution 2.02&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=GTP:GUANOSINE-5'-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36]
+|GENE= ARF6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ctxA, toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])
+}}
+'''Cholera toxin A1 subunit bound to its substrate, NAD+, and its human protein activator, ARF6'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-A5F is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=GTP:'>GTP</scene>, <scene name='pdbligand=NAD:'>NAD</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5F OCA].
+A5F is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5F OCA].
 ==Reference==
-Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16099990 16099990]
+Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16099990 16099990]
 [[Category: Homo sapiens]]
 [[Category: NAD(+)--diphthamide ADP-ribosyltransferase]]
@@ Line 26: / Line 35: @@
 [[Category: protein transport/transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:23:46 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:45:35 2008''