2a3p: Difference between revisions
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[[Image:2a3p.gif|left|200px]] | [[Image:2a3p.gif|left|200px]] | ||
'''Structure of Desulfovibrio desulfuricans G20 tetraheme cytochrome with bound molybdate''' | {{Structure | ||
|PDB= 2a3p |SIZE=350|CAPTION= <scene name='initialview01'>2a3p</scene>, resolution 2.300Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=MOO:MOLYBDATE ION'>MOO</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Structure of Desulfovibrio desulfuricans G20 tetraheme cytochrome with bound molybdate''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2A3P is a [ | 2A3P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3P OCA]. | ||
==Reference== | ==Reference== | ||
Desulfovibrio desulfuricans G20 tetraheme cytochrome structure at 1.5 Angstrom and cytochrome interaction with metal complexes., Pattarkine MV, Tanner JJ, Bottoms CA, Lee YH, Wall JD, J Mol Biol. 2006 May 19;358(5):1314-27. Epub 2006 Mar 23. PMID:[http:// | Desulfovibrio desulfuricans G20 tetraheme cytochrome structure at 1.5 Angstrom and cytochrome interaction with metal complexes., Pattarkine MV, Tanner JJ, Bottoms CA, Lee YH, Wall JD, J Mol Biol. 2006 May 19;358(5):1314-27. Epub 2006 Mar 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16580681 16580681] | ||
[[Category: Desulfovibrio desulfuricans]] | [[Category: Desulfovibrio desulfuricans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: tetraheme cytochrome]] | [[Category: tetraheme cytochrome]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:45:02 2008'' | ||
Revision as of 16:45, 20 March 2008
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Desulfovibrio desulfuricans G20 tetraheme cytochrome with bound molybdate
OverviewOverview
The structure of the type I tetraheme cytochrome c(3) from Desulfovibrio desulfuricans G20 was determined to 1.5 Angstrom by X-ray crystallography. In addition to the oxidized form, the structure of the molybdate-bound form of the protein was determined from oxidized crystals soaked in sodium molybdate. Only small structural shifts were obtained with metal binding, consistent with the remarkable structural stability of this protein. In vitro experiments with pure cytochrome showed that molybdate could oxidize the reduced cytochrome, although not as rapidly as U(VI) present as uranyl acetate. Alterations in the overall conformation and thermostability of the metal-oxidized protein were investigated by circular dichroism studies. Again, only small changes in protein structure were documented. The location of the molybdate ion near heme IV in the crystal structure suggested heme IV as the site of electron exit from the reduced cytochrome and implicated Lys14 and Lys56 in binding. Analysis of structurally conserved water molecules in type I cytochrome c(3) crystal structures identified interactions predicted to be important for protein stability and possibly for intramolecular electron transfer among heme molecules.
About this StructureAbout this Structure
2A3P is a Single protein structure of sequence from Desulfovibrio desulfuricans. Full crystallographic information is available from OCA.
ReferenceReference
Desulfovibrio desulfuricans G20 tetraheme cytochrome structure at 1.5 Angstrom and cytochrome interaction with metal complexes., Pattarkine MV, Tanner JJ, Bottoms CA, Lee YH, Wall JD, J Mol Biol. 2006 May 19;358(5):1314-27. Epub 2006 Mar 23. PMID:16580681
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