2a19: Difference between revisions
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[[Image:2a19.gif|left|200px]] | [[Image:2a19.gif|left|200px]] | ||
'''PKR kinase domain- eIF2alpha- AMP-PNP complex.''' | {{Structure | ||
|PDB= 2a19 |SIZE=350|CAPTION= <scene name='initialview01'>2a19</scene>, resolution 2.50Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER'>ANP</scene> | |||
|ACTIVITY= | |||
|GENE= SUI2, TIF211 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), PRKR, EIF2AK2, PKR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''PKR kinase domain- eIF2alpha- AMP-PNP complex.''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2A19 is a [ | 2A19 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A19 OCA]. | ||
==Reference== | ==Reference== | ||
Higher-order substrate recognition of eIF2alpha by the RNA-dependent protein kinase PKR., Dar AC, Dever TE, Sicheri F, Cell. 2005 Sep 23;122(6):887-900. PMID:[http:// | Higher-order substrate recognition of eIF2alpha by the RNA-dependent protein kinase PKR., Dar AC, Dever TE, Sicheri F, Cell. 2005 Sep 23;122(6):887-900. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16179258 16179258] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:44:10 2008'' | ||
Revision as of 16:44, 20 March 2008
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| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | SUI2, TIF211 (Saccharomyces cerevisiae), PRKR, EIF2AK2, PKR (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PKR kinase domain- eIF2alpha- AMP-PNP complex.
OverviewOverview
In response to binding viral double-stranded RNA byproducts within a cell, the RNA-dependent protein kinase PKR phosphorylates the alpha subunit of the translation initiation factor eIF2 on a regulatory site, Ser51. This triggers the general shutdown of protein synthesis and inhibition of viral propagation. To understand the basis for substrate recognition by and the regulation of PKR, we determined X-ray crystal structures of the catalytic domain of PKR in complex with eIF2alpha. The structures reveal that eIF2alpha binds to the C-terminal catalytic lobe while catalytic-domain dimerization is mediated by the N-terminal lobe. In addition to inducing a local unfolding of the Ser51 acceptor site in eIF2alpha, its mode of binding to PKR affords the Ser51 site full access to the catalytic cleft of PKR. The generality and implications of the structural mechanisms uncovered for PKR to the larger family of four human eIF2alpha protein kinases are discussed.
DiseaseDisease
Known disease associated with this structure: Kallmann syndrome 3 OMIM:[607123]
About this StructureAbout this Structure
2A19 is a Protein complex structure of sequences from Homo sapiens and Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Higher-order substrate recognition of eIF2alpha by the RNA-dependent protein kinase PKR., Dar AC, Dever TE, Sicheri F, Cell. 2005 Sep 23;122(6):887-900. PMID:16179258
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