2ro5: Difference between revisions
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ro5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ro5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ro5 RCSB], [http://www.ebi.ac.uk/pdbsum/2ro5 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ro5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ro5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ro5 RCSB], [http://www.ebi.ac.uk/pdbsum/2ro5 PDBsum]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/SP5T_BACSU SP5T_BACSU]] Positive and negative transcriptional regulator of sigma G-dependent genes. May provide a mechanism of feedback control that is important for forespore development. | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Bobay, B G | [[Category: Bobay, B G]] | ||
[[Category: Cavanagh, J | [[Category: Cavanagh, J]] | ||
[[Category: Kojetin, D J | [[Category: Kojetin, D J]] | ||
[[Category: Rance, M | [[Category: Rance, M]] | ||
[[Category: Strauch, M A | [[Category: Strauch, M A]] | ||
[[Category: Sullivan, D M | [[Category: Sullivan, D M]] | ||
[[Category: Thompson, R J | [[Category: Thompson, R J]] | ||
[[Category: Activator]] | [[Category: Activator]] | ||
[[Category: Dna-binding]] | [[Category: Dna-binding]] | ||
Revision as of 00:41, 25 December 2014
RDC-refined solution structure of the N-terminal DNA recognition domain of the Bacillus subtilis transition-state regulator SpoVTRDC-refined solution structure of the N-terminal DNA recognition domain of the Bacillus subtilis transition-state regulator SpoVT
Structural highlights
Function[SP5T_BACSU] Positive and negative transcriptional regulator of sigma G-dependent genes. May provide a mechanism of feedback control that is important for forespore development. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedUnderstanding the DNA recognition and binding by the AbrB-like family of transcriptional regulators is of significant interest since these proteins enable bacteria to elicit the appropriate response to diverse environmental stimuli. Although these "transition-state regulator" proteins have been well characterized at the genetic level, the general and specific mechanisms of DNA binding remain elusive. We present RDC-refined NMR solution structures and dynamic properties of the DNA-binding domains of three Bacillus subtilis transition-state regulators: AbrB, Abh, and SpoVT. We combined previously investigated DNase I footprinting, DNA methylation, gel-shift assays, and mutagenic and NMR studies to generate a structural model of the complex between AbrBN(55) and its cognate promoter, abrB8. These investigations have enabled us to generate a model for the specific nature of the transition-state regulator-DNA interaction, a structure that has remained elusive thus far. Insights into the nature of DNA binding of AbrB-like transcription factors.,Sullivan DM, Bobay BG, Kojetin DJ, Thompson RJ, Rance M, Strauch MA, Cavanagh J Structure. 2008 Nov 12;16(11):1702-13. PMID:19000822[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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