1zy7: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1zy7.gif|left|200px]] | [[Image:1zy7.gif|left|200px]] | ||
'''Crystal structure of the catalytic domain of an adenosine deaminase that acts on RNA (hADAR2) bound to inositol hexakisphosphate (IHP)''' | {{Structure | ||
|PDB= 1zy7 |SIZE=350|CAPTION= <scene name='initialview01'>1zy7</scene>, resolution 1.70Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=IHP:INOSITOL HEXAKISPHOSPHATE'>IHP</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of the catalytic domain of an adenosine deaminase that acts on RNA (hADAR2) bound to inositol hexakisphosphate (IHP)''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1ZY7 is a [ | 1ZY7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZY7 OCA]. | ||
==Reference== | ==Reference== | ||
Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editing., Macbeth MR, Schubert HL, Vandemark AP, Lingam AT, Hill CP, Bass BL, Science. 2005 Sep 2;309(5740):1534-9. PMID:[http:// | Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editing., Macbeth MR, Schubert HL, Vandemark AP, Lingam AT, Hill CP, Bass BL, Science. 2005 Sep 2;309(5740):1534-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16141067 16141067] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 26: | Line 35: | ||
[[Category: zinc coordination]] | [[Category: zinc coordination]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:41:17 2008'' | ||
Revision as of 16:41, 20 March 2008
| |||||||
| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the catalytic domain of an adenosine deaminase that acts on RNA (hADAR2) bound to inositol hexakisphosphate (IHP)
OverviewOverview
We report the crystal structure of the catalytic domain of human ADAR2, an RNA editing enzyme, at 1.7 angstrom resolution. The structure reveals a zinc ion in the active site and suggests how the substrate adenosine is recognized. Unexpectedly, inositol hexakisphosphate (IP6) is buried within the enzyme core, contributing to the protein fold. Although there are no reports that adenosine deaminases that act on RNA (ADARs) require a cofactor, we show that IP6 is required for activity. Amino acids that coordinate IP6 in the crystal structure are conserved in some adenosine deaminases that act on transfer RNA (tRNA) (ADATs), related enzymes that edit tRNA. Indeed, IP6 is also essential for in vivo and in vitro deamination of adenosine 37 of tRNAala by ADAT1.
About this StructureAbout this Structure
1ZY7 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editing., Macbeth MR, Schubert HL, Vandemark AP, Lingam AT, Hill CP, Bass BL, Science. 2005 Sep 2;309(5740):1534-9. PMID:16141067
Page seeded by OCA on Thu Mar 20 15:41:17 2008