1zxa: Difference between revisions
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[[Image:1zxa.gif|left|200px]] | [[Image:1zxa.gif|left|200px]] | ||
'''Solution Structure of the Coiled-Coil Domain of cGMP-dependent Protein Kinase Ia''' | {{Structure | ||
|PDB= 1zxa |SIZE=350|CAPTION= <scene name='initialview01'>1zxa</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] | |||
|GENE= PRKG1, PRKGR1A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''Solution Structure of the Coiled-Coil Domain of cGMP-dependent Protein Kinase Ia''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1ZXA is a [ | 1ZXA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZXA OCA]. | ||
==Reference== | ==Reference== | ||
Rapid and accurate structure determination of coiled-coil domains using NMR dipolar couplings: application to cGMP-dependent protein kinase Ialpha., Schnell JR, Zhou GP, Zweckstetter M, Rigby AC, Chou JJ, Protein Sci. 2005 Sep;14(9):2421-8. PMID:[http:// | Rapid and accurate structure determination of coiled-coil domains using NMR dipolar couplings: application to cGMP-dependent protein kinase Ialpha., Schnell JR, Zhou GP, Zweckstetter M, Rigby AC, Chou JJ, Protein Sci. 2005 Sep;14(9):2421-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16131665 16131665] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Non-specific serine/threonine protein kinase]] | [[Category: Non-specific serine/threonine protein kinase]] | ||
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[[Category: parallel coiled coil dimer]] | [[Category: parallel coiled coil dimer]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:40:55 2008'' | ||
Revision as of 16:40, 20 March 2008
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| Gene: | PRKG1, PRKGR1A (Homo sapiens) | ||||||
| Activity: | Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of the Coiled-Coil Domain of cGMP-dependent Protein Kinase Ia
OverviewOverview
Coiled-coil motifs play essential roles in protein assembly and molecular recognition, and are therefore the targets of many ongoing structural and functional studies. However, owing to the dynamic nature of many of the smaller coiled-coil domains, crystallization for X-ray studies is very challenging. Determination of elongated structures using standard NMR approaches is inefficient and usually yields low-resolution structures due to accumulation of small errors over long distances. Here we describe a solution NMR approach based on residual dipolar couplings (RDCs) for rapid and accurate structure determination of coiled-coil dimers. Using this approach, we were able to determine the high-resolution structure of the coiled-coil domain of cGMP-dependent protein kinase Ialpha, a protein of previously unknown structure that is critical for physiological relaxation of vascular smooth muscle. This approach can be extended to solve coiled-coil structures with higher order assemblies.
About this StructureAbout this Structure
1ZXA is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Rapid and accurate structure determination of coiled-coil domains using NMR dipolar couplings: application to cGMP-dependent protein kinase Ialpha., Schnell JR, Zhou GP, Zweckstetter M, Rigby AC, Chou JJ, Protein Sci. 2005 Sep;14(9):2421-8. PMID:16131665
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