1yxq: Difference between revisions

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Revision as of 16:28, 20 March 2008

File:1yxq.gif

, resolution 2.01Å

Ligands:

, , and

Coordinates:

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Crystal structure of actin in complex with swinholide A

OverviewOverview

Marine toxins targeting the actin cytoskeleton represent a new and promising class of anti-cancer compounds. Here we present a 2.0 A resolution structure of swinholide A, a marine macrolide, bound to two actin molecules. The structure demonstrates that the actin dimer in the complex does not represent a physiologically relevant entity, for the two actin molecules do not interact with each other. The swinholide A actin binding site is the same as that targeted by toxins of the trisoxazole family and numerous actin binding proteins, highlighting the importance of this site in actin polymerization. The observed structure reveals the mechanism of action of swinholide A and provides a structural framework about which to design new agents directed at the cytoskeleton.

About this StructureAbout this Structure

1YXQ is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of swinholide A binding to actin., Klenchin VA, King R, Tanaka J, Marriott G, Rayment I, Chem Biol. 2005 Mar;12(3):287-91. PMID:15797212

Page seeded by OCA on Thu Mar 20 15:28:47 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1yxq.gif|left|200px]]<br /><applet load="1yxq" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1yxq.gif|left|200px]]
-caption="1yxq, resolution 2.01&Aring;" />
-'''Crystal structure of actin in complex with swinholide A'''<br />
+ {{Structure
+|PDB= 1yxq |SIZE=350|CAPTION= <scene name='initialview01'>1yxq</scene>, resolution 2.01&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=SWI:SWINHOLIDE+A'>SWI</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Crystal structure of actin in complex with swinholide A'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-YXQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ATP:'>ATP</scene>, <scene name='pdbligand=SWI:'>SWI</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YXQ OCA].
+YXQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YXQ OCA].
 ==Reference==
-Structural basis of swinholide A binding to actin., Klenchin VA, King R, Tanaka J, Marriott G, Rayment I, Chem Biol. 2005 Mar;12(3):287-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15797212 15797212]
+Structural basis of swinholide A binding to actin., Klenchin VA, King R, Tanaka J, Marriott G, Rayment I, Chem Biol. 2005 Mar;12(3):287-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15797212 15797212]
 [[Category: Oryctolagus cuniculus]]
 [[Category: Single protein]]
@@ Line 24: / Line 33: @@
 [[Category: actin; swinholide a; macrolide toxin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:10:10 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:28:47 2008''