1uuw: Difference between revisions

Nitric oxide (NO) is commonly used as an analogue for dioxygen in, structural and spectroscopic studies of oxygen binding and oxygen, activation. In this study, crystallographic structures of naphthalene, dioxygenase (NDO) in complex with nitric oxide are reported. In the, presence of the aromatic substrate indole, NO is bound end-on to the, active-site mononuclear iron of NDO. The structural observations correlate, well with spectroscopic measurements of NO binding to NDO in solution., However, the end-on binding of NO is in contrast to the recently reported, structure of oxygen to the active-site iron of NDO that binds side-on., While NO is a good oxygen analogue with many similarities to O(2), the, different binding mode of NO to the active-site iron atom leads to, different mechanistic implications. Hence, caution needs to be used in, extrapolating NO as an analogue to O(2) binding.

1UUW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with FE, SO4, FES and NO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Naphthalene_1,2-dioxygenase Naphthalene 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.12 1.14.12.12] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UUW OCA].  

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