1ylk: Difference between revisions

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Revision as of 16:24, 20 March 2008

File:1ylk.gif

, resolution 2.00Å

Ligands:

and

Gene:

Rv1284 (Mycobacterium tuberculosis H37Rv)

Coordinates:

save as pdb, mmCIF, xml

Crystal Structure of Rv1284 from Mycobacterium tuberculosis in Complex with Thiocyanate

OverviewOverview

Carbonic anhydrases catalyze the reversible hydration of carbon dioxide to form bicarbonate. This activity is universally required for fatty acid biosynthesis as well as for the production of a number of small molecules, pH homeostasis, and other functions. At least three different carbonic anhydrase families are known to exist, of which the alpha-class found in humans has been studied in most detail. In the present work, we describe the structures of two of the three beta-class carbonic anhydrases that have been identified in Mycobacterium tuberculosis, i.e. Rv1284 and Rv3588c. Both structures were solved by molecular replacement and then refined to resolutions of 2.0 and 1.75 A, respectively. The active site of Rv1284 is small and almost completely shielded from solvent, whereas that of Rv3588c is larger and quite open to solution. Differences in coordination of the active site metal are also observed. In Rv3588c, an aspartic acid side chain displaces a water molecule and coordinates directly to the zinc ion, thereby closing the zinc coordination sphere and breaking the salt link to a nearby arginine that is a feature of Rv1284. The two carbonic anhydrases thus exhibit both of the metal coordination geometries that have previously been observed for structures in this family. Activity studies demonstrate that Rv3588c is a completely functional carbonic anhydrase. The apparent lack of activity of Rv1284 in the present assay system is likely exacerbated by the observed depletion of zinc in the preparation.

About this StructureAbout this Structure

1YLK is a Single protein structure of sequence from Mycobacterium tuberculosis h37rv. Full crystallographic information is available from OCA.

ReferenceReference

Structure and function of carbonic anhydrases from Mycobacterium tuberculosis., Suarez Covarrubias A, Larsson AM, Hogbom M, Lindberg J, Bergfors T, Bjorkelid C, Mowbray SL, Unge T, Jones TA, J Biol Chem. 2005 May 13;280(19):18782-9. Epub 2005 Mar 6. PMID:15753099

Page seeded by OCA on Thu Mar 20 15:24:26 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1ylk.gif|left|200px]]<br /><applet load="1ylk" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ylk.gif|left|200px]]
-caption="1ylk, resolution 2.00&Aring;" />
-'''Crystal Structure of Rv1284 from Mycobacterium tuberculosis in Complex with Thiocyanate'''<br />
+ {{Structure
+|PDB= 1ylk |SIZE=350|CAPTION= <scene name='initialview01'>1ylk</scene>, resolution 2.00&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=SCN:THIOCYANATE ION'>SCN</scene>
+|ACTIVITY=
+|GENE= Rv1284 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 Mycobacterium tuberculosis H37Rv])
+}}
+'''Crystal Structure of Rv1284 from Mycobacterium tuberculosis in Complex with Thiocyanate'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-YLK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=SCN:'>SCN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YLK OCA].
+YLK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YLK OCA].
 ==Reference==
-Structure and function of carbonic anhydrases from Mycobacterium tuberculosis., Suarez Covarrubias A, Larsson AM, Hogbom M, Lindberg J, Bergfors T, Bjorkelid C, Mowbray SL, Unge T, Jones TA, J Biol Chem. 2005 May 13;280(19):18782-9. Epub 2005 Mar 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15753099 15753099]
+Structure and function of carbonic anhydrases from Mycobacterium tuberculosis., Suarez Covarrubias A, Larsson AM, Hogbom M, Lindberg J, Bergfors T, Bjorkelid C, Mowbray SL, Unge T, Jones TA, J Biol Chem. 2005 May 13;280(19):18782-9. Epub 2005 Mar 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15753099 15753099]
 [[Category: Mycobacterium tuberculosis h37rv]]
 [[Category: Single protein]]
@@ Line 29: / Line 38: @@
 [[Category: rv1284]]
 [[Category: spine]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
 [[Category: structural proteomics in europe]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:06:38 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:24:26 2008''