1ylb: Difference between revisions
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[[Image:1ylb.gif|left|200px]] | [[Image:1ylb.gif|left|200px]] | ||
'''NMR solution structure of the reduced spinach plastocyanin''' | {{Structure | ||
|PDB= 1ylb |SIZE=350|CAPTION= <scene name='initialview01'>1ylb</scene> | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CU1:COPPER (I) ION'>CU1</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''NMR solution structure of the reduced spinach plastocyanin''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1YLB is a [ | 1YLB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YLB OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the intermolecular complex between plastocyanin and cytochrome f from spinach., Musiani F, Dikiy A, Semenov AY, Ciurli S, J Biol Chem. 2005 May 13;280(19):18833-41. Epub 2005 Feb 3. PMID:[http:// | Structure of the intermolecular complex between plastocyanin and cytochrome f from spinach., Musiani F, Dikiy A, Semenov AY, Ciurli S, J Biol Chem. 2005 May 13;280(19):18833-41. Epub 2005 Feb 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15691836 15691836] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Spinacia oleracea]] | [[Category: Spinacia oleracea]] | ||
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[[Category: spinach]] | [[Category: spinach]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:24:25 2008'' | ||
Revision as of 16:24, 20 March 2008
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NMR solution structure of the reduced spinach plastocyanin
OverviewOverview
In oxygenic photosynthesis, plastocyanin shuttles electrons between the membrane-bound complexes cytochrome b6f and photosystem I. The homologous complex between cytochrome f and plastocyanin, both from spinach, is the object of this study. The solution structure of the reduced spinach plastocyanin was determined using high field NMR spectroscopy, whereas the model structure of oxidized cytochrome f was obtained by homology modeling calculations and molecular dynamics. The model structure of the intermolecular complex was calculated using the program AUTODOCK, taking into account biological information obtained from mutagenesis experiments. The best electron transfer pathway from the heme group of cytochrome f to the copper ion of plastocyanin was calculated using the program HARLEM, obtaining a coupling decay value of 1.8 x 10(-4). Possible mechanisms of interaction and electron transfer between plastocyanin and cytochrome f were discussed considering the possible formation of a supercomplex that associates one cytochrome b6f, one photosystem I, and one plastocyanin.
About this StructureAbout this Structure
1YLB is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the intermolecular complex between plastocyanin and cytochrome f from spinach., Musiani F, Dikiy A, Semenov AY, Ciurli S, J Biol Chem. 2005 May 13;280(19):18833-41. Epub 2005 Feb 3. PMID:15691836
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