1yk9: Difference between revisions

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Revision as of 16:23, 20 March 2008

File:1yk9.gif

, resolution 2.70Å

Gene:

Rv1625c (Mycobacterium tuberculosis)

Activity:

Adenylate cyclase, with EC number 4.6.1.1

Coordinates:

save as pdb, mmCIF, xml

Crystal structure of a mutant form of the mycobacterial adenylyl cyclase Rv1625c

OverviewOverview

The Rv1625c Class III adenylyl cyclase from Mycobacterium tuberculosis is a homodimeric enzyme with two catalytic centers at the dimer interface, and shows sequence similarity with the mammalian adenylyl and guanylyl cyclases. Mutation of the substrate-specifying residues in the catalytic domain of Rv1625c, either independently or together, to those present in guanylyl cyclases not only failed to confer guanylyl cyclase activity to the protein, but also severely abrogated the adenylyl cyclase activity of the enzyme. Biochemical analysis revealed alterations in the behavior of the mutants on ion-exchange chromatography, indicating differences in the surface-exposed charge upon mutation of substrate-specifying residues. The mutant proteins showed alterations in oligomeric status as compared to the wild-type enzyme, and differing abilities to heterodimerize with the wild-type protein. The crystal structure of a mutant has been solved to a resolution of 2.7A. On the basis of the structure, and additional biochemical studies, we provide possible reasons for the altered properties of the mutant proteins, as well as highlight unique structural features of the Rv1625c adenylyl cyclase.

About this StructureAbout this Structure

1YK9 is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

A structural basis for the role of nucleotide specifying residues in regulating the oligomerization of the Rv1625c adenylyl cyclase from M. tuberculosis., Ketkar AD, Shenoy AR, Ramagopal UA, Visweswariah SS, Suguna K, J Mol Biol. 2006 Mar 3;356(4):904-16. Epub 2005 Dec 22. PMID:16403515

Page seeded by OCA on Thu Mar 20 15:23:58 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1yk9.gif|left|200px]]<br /><applet load="1yk9" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1yk9.gif|left|200px]]
-caption="1yk9, resolution 2.70&Aring;" />
-'''Crystal structure of a mutant form of the mycobacterial adenylyl cyclase Rv1625c'''<br />
+ {{Structure
+|PDB= 1yk9 |SIZE=350|CAPTION= <scene name='initialview01'>1yk9</scene>, resolution 2.70&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY= [http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1]
+|GENE= Rv1625c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
+}}
+'''Crystal structure of a mutant form of the mycobacterial adenylyl cyclase Rv1625c'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-YK9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Active as [http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YK9 OCA].
+YK9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YK9 OCA].
 ==Reference==
-A structural basis for the role of nucleotide specifying residues in regulating the oligomerization of the Rv1625c adenylyl cyclase from M. tuberculosis., Ketkar AD, Shenoy AR, Ramagopal UA, Visweswariah SS, Suguna K, J Mol Biol. 2006 Mar 3;356(4):904-16. Epub 2005 Dec 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16403515 16403515]
+A structural basis for the role of nucleotide specifying residues in regulating the oligomerization of the Rv1625c adenylyl cyclase from M. tuberculosis., Ketkar AD, Shenoy AR, Ramagopal UA, Visweswariah SS, Suguna K, J Mol Biol. 2006 Mar 3;356(4):904-16. Epub 2005 Dec 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16403515 16403515]
 [[Category: Adenylate cyclase]]
 [[Category: Mycobacterium tuberculosis]]
@@ Line 23: / Line 32: @@
 [[Category: protein structure initiative]]
 [[Category: psi]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
 [[Category: tb structural genomics consortium]]
 [[Category: tbsgc]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:06:14 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:23:58 2008''