1ycd: Difference between revisions
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[[Image:1ycd.gif|left|200px]] | [[Image:1ycd.gif|left|200px]] | ||
'''Crystal structure of yeast FSH1/YHR049W, a member of the serine hydrolase family''' | {{Structure | ||
|PDB= 1ycd |SIZE=350|CAPTION= <scene name='initialview01'>1ycd</scene>, resolution 1.70Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=LI5:2-HYDROXY-4,5-DIOXOHEPTYL HYDROGEN PHOSPHONATE'>LI5</scene> | |||
|ACTIVITY= | |||
|GENE= YHR049w ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |||
}} | |||
'''Crystal structure of yeast FSH1/YHR049W, a member of the serine hydrolase family''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1YCD is a [ | 1YCD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YCD OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of yeast YHR049W/FSH1, a member of the serine hydrolase family., Quevillon-Cheruel S, Leulliot N, Graille M, Hervouet N, Coste F, Benedetti H, Zelwer C, Janin J, Van Tilbeurgh H, Protein Sci. 2005 May;14(5):1350-6. Epub 2005 Mar 31. PMID:[http:// | Crystal structure of yeast YHR049W/FSH1, a member of the serine hydrolase family., Quevillon-Cheruel S, Leulliot N, Graille M, Hervouet N, Coste F, Benedetti H, Zelwer C, Janin J, Van Tilbeurgh H, Protein Sci. 2005 May;14(5):1350-6. Epub 2005 Mar 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15802654 15802654] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: esterase]] | [[Category: esterase]] | ||
[[Category: lipase]] | [[Category: lipase]] | ||
[[Category: paris-sud yeast structural | [[Category: paris-sud yeast structural genomic]] | ||
[[Category: s. cerevisiae]] | [[Category: s. cerevisiae]] | ||
[[Category: serine hydrolase]] | [[Category: serine hydrolase]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
[[Category: ysg]] | [[Category: ysg]] | ||
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Revision as of 16:21, 20 March 2008
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| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | YHR049w (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of yeast FSH1/YHR049W, a member of the serine hydrolase family
OverviewOverview
Yhr049w/FSH1 was recently identified in a combined computational and experimental proteomics analysis for the detection of active serine hydrolases in yeast. This analysis suggested that FSH1 might be a serine-type hydrolase belonging to the broad functional alphabeta-hydrolase superfamily. In order to get insight into the molecular function of this gene, it was targeted in our yeast structural genomics project. The crystal structure of the protein confirms that it contains a Ser/His/Asp catalytic triad that is part of a minimal alpha/beta-hydrolase fold. The architecture of the putative active site and analogies with other protein structures suggest that FSH1 may be an esterase. This finding was further strengthened by the unexpected presence of a compound covalently bound to the catalytic serine in the active site. Apparently, the enzyme was trapped with a reactive compound during the purification process.
About this StructureAbout this Structure
1YCD is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of yeast YHR049W/FSH1, a member of the serine hydrolase family., Quevillon-Cheruel S, Leulliot N, Graille M, Hervouet N, Coste F, Benedetti H, Zelwer C, Janin J, Van Tilbeurgh H, Protein Sci. 2005 May;14(5):1350-6. Epub 2005 Mar 31. PMID:15802654
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