2oz9: Difference between revisions
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==Overview== | ==Overview== | ||
An orthorhombic crystal form of trp repressor (aporepressor plus, L-tryptophan ligand) was solved by molecular replacement, refined to 1.65, A resolution, and compared to the structure of the repressor in trigonal, crystals. Even though these two crystal forms of repressor were grown, under identical conditions, the refined structures have distinctly, different conformations of the DNA-binding domains. Unlike the, repressor/aporepressor structural transition, the conformational shift is, not caused by the binding or loss of the L-tryptophan ligand. We conclude, that while L-tryptophan binding is essential for forming a specific, complex with trp operator DNA, the corepressor ligand does not lock the, repressor into a single conformation that is complementary to the, operator. This . | An orthorhombic crystal form of trp repressor (aporepressor plus, L-tryptophan ligand) was solved by molecular replacement, refined to 1.65, A resolution, and compared to the structure of the repressor in trigonal, crystals. Even though these two crystal forms of repressor were grown, under identical conditions, the refined structures have distinctly, different conformations of the DNA-binding domains. Unlike the, repressor/aporepressor structural transition, the conformational shift is, not caused by the binding or loss of the L-tryptophan ligand. We conclude, that while L-tryptophan binding is essential for forming a specific, complex with trp operator DNA, the corepressor ligand does not lock the, repressor into a single conformation that is complementary to the, operator. This flexibility may be required by the various binding modes, proposed for trp repressor in its search for and adherence to its three, different operator sites. | ||
==About this Structure== | ==About this Structure== | ||
2OZ9 is a | 2OZ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with NA, SO4 and TRP as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 2WRP. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2OZ9 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: dna binding regulatory protein]] | [[Category: dna binding regulatory protein]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:09:35 2007'' | ||