4kyt: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==The structure of superinhibitory phospholamban bound to the calcium pump SERCA1a== | |||
<StructureSection load='4kyt' size='340' side='right' caption='[[4kyt]], [[Resolution|resolution]] 2.83Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4kyt]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Canfa Canfa] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KYT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KYT FirstGlance]. <br> | |||
==Function== | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PLN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9615 CANFA])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kyt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kyt RCSB], [http://www.ebi.ac.uk/pdbsum/4kyt PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/PPLA_CANFA PPLA_CANFA]] Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. | [[http://www.uniprot.org/uniprot/PPLA_CANFA PPLA_CANFA]] Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
P-type ATPases are a large family of enzymes that actively transport ions across biological membranes by interconverting between high (E1) and low (E2) ion-affinity states; these transmembrane transporters carry out critical processes in nearly all forms of life. In striated muscle, the archetype P-type ATPase, SERCA (sarco(endo)plasmic reticulum Ca2+-ATPase), pumps contractile-dependent Ca2+ ions into the lumen of sarcoplasmic reticulum, which initiates myocyte relaxation and refills the sarcoplasmic reticulum in preparation for the next contraction. In cardiac muscle, SERCA is regulated by phospholamban (PLB), a small inhibitory phosphoprotein that decreases the Ca2+ affinity of SERCA and attenuates contractile strength. cAMP-dependent phosphorylation of PLB reverses Ca2+-ATPase inhibition with powerful contractile effects. Here we present the long sought crystal structure of the PLB/SERCA complex at 2.8 A resolution. The structure was solved in the absence of Ca2+ in a novel detergent system employing alkyl mannosides. The structure shows PLB bound to a previously undescribed conformation of SERCA in which the Ca2+ binding sites are collapsed and devoid of divalent cations (E2-PLB). This new structure represents one of the key unsolved conformational states of SERCA and provides a structural explanation for how dephosphorylated PLB decreases Ca2+ affinity and depresses cardiac contractility. | |||
The Structural Basis for Phospholamban Inhibition of the Calcium Pump in Sarcoplasmic Reticulum.,Akin BL, Hurley TD, Chen Z, Jones LR J Biol Chem. 2013 Aug 31. PMID:23996003<ref>PMID:23996003</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Canfa]] | [[Category: Canfa]] | ||
[[Category: Oryctolagus cuniculus]] | [[Category: Oryctolagus cuniculus]] | ||
[[Category: Akin, B L | [[Category: Akin, B L]] | ||
[[Category: Hurley, T D | [[Category: Hurley, T D]] | ||
[[Category: Jones, L R | [[Category: Jones, L R]] | ||
[[Category: Calcium pump]] | [[Category: Calcium pump]] | ||
[[Category: Membrane]] | [[Category: Membrane]] | ||