1xxd: Difference between revisions
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[[Image:1xxd.gif|left|200px]] | [[Image:1xxd.gif|left|200px]] | ||
'''Crystal Structure of the FXIa Catalytic Domain in Complex with mutated Ecotin''' | {{Structure | ||
|PDB= 1xxd |SIZE=350|CAPTION= <scene name='initialview01'>1xxd</scene>, resolution 2.91Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Coagulation_factor_XIa Coagulation factor XIa], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.27 3.4.21.27] | |||
|GENE= F11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), eco,eti ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Crystal Structure of the FXIa Catalytic Domain in Complex with mutated Ecotin''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1XXD is a [ | 1XXD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XXD OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structures of the FXIa catalytic domain in complex with ecotin mutants reveal substrate-like interactions., Jin L, Pandey P, Babine RE, Gorga JC, Seidl KJ, Gelfand E, Weaver DT, Abdel-Meguid SS, Strickler JE, J Biol Chem. 2005 Feb 11;280(6):4704-12. Epub 2004 Nov 15. PMID:[http:// | Crystal structures of the FXIa catalytic domain in complex with ecotin mutants reveal substrate-like interactions., Jin L, Pandey P, Babine RE, Gorga JC, Seidl KJ, Gelfand E, Weaver DT, Abdel-Meguid SS, Strickler JE, J Biol Chem. 2005 Feb 11;280(6):4704-12. Epub 2004 Nov 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15545266 15545266] | ||
[[Category: Coagulation factor XIa]] | [[Category: Coagulation factor XIa]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: fxia; catalytic domain; serine protein; ecotin]] | [[Category: fxia; catalytic domain; serine protein; ecotin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:15:43 2008'' | ||
Revision as of 16:15, 20 March 2008
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| , resolution 2.91Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | F11 (Homo sapiens), eco,eti (Escherichia coli) | ||||||
| Activity: | Coagulation factor XIa, with EC number 3.4.21.27 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the FXIa Catalytic Domain in Complex with mutated Ecotin
OverviewOverview
Thrombosis can lead to life-threatening conditions such as acute myocardial infarction, pulmonary embolism, and stroke. Although commonly used anti-coagulant drugs, such as low molecular weight heparin and warfarin, are effective, they carry a significant risk of inducing severe bleeding complications, and there is a need for safer drugs. Activated Factor XI (FXIa) is a key enzyme in the amplification phase of the coagulation cascade. Anti-human FXI antibody significantly reduces thrombus growth in a baboon thrombosis model without bleeding problems (Gruber, A., and Hanson, S. R. (2003) Blood 102, 953-955). Therefore, FXIa is a potential target for anti-thrombosis therapy. To determine the structure of FXIa, we derived a recombinant catalytic domain of FXI, consisting of residues 370-607 (rhFXI370-607). Here we report the first crystal structure of rhFXI370-607 in complex with a substitution mutant of ecotin, a panserine protease protein inhibitor secreted by Escherichia coli, to 2.2 A resolution. The presence of ecotin not only assisted in the crystallization of the enzyme but also revealed unique structural features in the active site of FXIa. Subsequently, the sequence from P5 to P2' in ecotin was mutated to the FXIa substrate sequence, and the structures of the rhFXI370-607-ecotin mutant complexes were determined. These structures provide us with an understanding of substrate binding interactions of FXIa, the structural information essential for the structure-based design of FXIa-selective inhibitors.
DiseaseDisease
Known diseases associated with this structure: Factor XI deficiency, autosomal dominant OMIM:[264900], Factor XI deficiency, autosomal recessive OMIM:[264900]
About this StructureAbout this Structure
1XXD is a Protein complex structure of sequences from Escherichia coli and Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of the FXIa catalytic domain in complex with ecotin mutants reveal substrate-like interactions., Jin L, Pandey P, Babine RE, Gorga JC, Seidl KJ, Gelfand E, Weaver DT, Abdel-Meguid SS, Strickler JE, J Biol Chem. 2005 Feb 11;280(6):4704-12. Epub 2004 Nov 15. PMID:15545266
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