1xmt: Difference between revisions

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Revision as of 16:11, 20 March 2008

File:1xmt.gif

, resolution 1.150Å

Ligands:

Gene:

At1g77540 (Arabidopsis thaliana)

Coordinates:

save as pdb, mmCIF, xml

X-ray structure of gene product from arabidopsis thaliana at1g77540

OverviewOverview

We describe X-ray crystal and NMR solution structures of the protein coded for by Arabidopsis thaliana gene At1g77540.1 (At1g77540). The crystal structure was determined to 1.15 A with an R factor of 14.9% (Rfree = 17.0%) by multiple-wavelength anomalous diffraction using sodium bromide derivatized crystals. The ensemble of NMR conformers was determined with protein samples labeled with 15N and 13C + 15N. The X-ray structure and NMR ensemble were closely similar with rmsd 1.4 A for residues 8-93. At1g77540 was found to adopt a fold similar to that of GCN5-related N-acetyltransferases. Enzymatic activity assays established that At1g77540 possesses weak acetyltransferase activity against histones H3 and H4. Chemical shift perturbations observed in 15N-HSQC spectra upon the addition of CoA indicated that the cofactor binds and identified its binding site. The molecular details of this interaction were further elucidated by solving the X-ray structure of the At1g77540-CoA complex. This work establishes that the domain family COG2388 represents a novel class of acetyltransferase and provides insight into possible mechanistic roles of the conserved Cys76 and His41 residues of this family.

About this StructureAbout this Structure

1XMT is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

ReferenceReference

Structure of Arabidopsis thaliana At1g77540 protein, a minimal acetyltransferase from the COG2388 family., Tyler RC, Bitto E, Berndsen CE, Bingman CA, Singh S, Lee MS, Wesenberg GE, Denu JM, Phillips GN Jr, Markley JL, Biochemistry. 2006 Dec 5;45(48):14325-36. PMID:17128971

Page seeded by OCA on Thu Mar 20 15:11:55 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1xmt.gif|left|200px]]<br /><applet load="1xmt" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1xmt.gif|left|200px]]
-caption="1xmt, resolution 1.150&Aring;" />
-'''X-ray structure of gene product from arabidopsis thaliana at1g77540'''<br />
+ {{Structure
+|PDB= 1xmt |SIZE=350|CAPTION= <scene name='initialview01'>1xmt</scene>, resolution 1.150&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=BR:BROMIDE ION'>BR</scene>
+|ACTIVITY=
+|GENE= At1g77540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])
+}}
+'''X-ray structure of gene product from arabidopsis thaliana at1g77540'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-XMT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with <scene name='pdbligand=BR:'>BR</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XMT OCA].
+XMT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XMT OCA].
 ==Reference==
-Structure of Arabidopsis thaliana At1g77540 protein, a minimal acetyltransferase from the COG2388 family., Tyler RC, Bitto E, Berndsen CE, Bingman CA, Singh S, Lee MS, Wesenberg GE, Denu JM, Phillips GN Jr, Markley JL, Biochemistry. 2006 Dec 5;45(48):14325-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17128971 17128971]
+Structure of Arabidopsis thaliana At1g77540 protein, a minimal acetyltransferase from the COG2388 family., Tyler RC, Bitto E, Berndsen CE, Bingman CA, Singh S, Lee MS, Wesenberg GE, Denu JM, Phillips GN Jr, Markley JL, Biochemistry. 2006 Dec 5;45(48):14325-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17128971 17128971]
 [[Category: Arabidopsis thaliana]]
 [[Category: Single protein]]
@@ Line 22: / Line 31: @@
 [[Category: BR]]
 [[Category: at1g77540]]
-[[Category: center for eukaryotic structural genomics]]
+[[Category: center for eukaryotic structural genomic]]
 [[Category: cesg]]
 [[Category: protein structure initiative]]
 [[Category: psi]]
 [[Category: putative acetyltransferase]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:56:31 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:11:55 2008''