4br2: Difference between revisions

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-{{STRUCTURE_4br2|  PDB=4br2  |  SCENE=  }}
+==rat NTPDase2 in complex with Ca UMPPNP==
-===rat NTPDase2 in complex with Ca UMPPNP===
+<StructureSection load='4br2' size='340' side='right' caption='[[4br2]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-{{ABSTRACT_PUBMED_23830739}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4br2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BR2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BR2 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=UNP:5-O-[(R)-HYDROXY{[(S)-HYDROXY(PHOSPHONOAMINO)PHOSPHORYL]OXY}PHOSPHORYL]URIDINE'>UNP</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bqz|4bqz]], [[4br0|4br0]], [[4br4|4br4]], [[4br5|4br5]], [[4br7|4br7]], [[4br9|4br9]], [[4bra|4bra]], [[4brc|4brc]], [[4brd|4brd]], [[4bre|4bre]], [[4brf|4brf]], [[4brg|4brg]], [[4brh|4brh]], [[4bri|4bri]], [[4brk|4brk]], [[4brl|4brl]], [[4brm|4brm]], [[4brn|4brn]], [[4bro|4bro]], [[4brp|4brp]], [[4brq|4brq]], [[3cj1|3cj1]], [[3cj7|3cj7]], [[3cj9|3cj9]], [[3cja|3cja]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4br2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4br2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4br2 RCSB], [http://www.ebi.ac.uk/pdbsum/4br2 PDBsum]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/ENTP2_RAT ENTP2_RAT]] In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+In vertebrates, membrane-bound ecto-nucleoside triphosphate diphosphohydrolases (NTPDases) on the cell surface are responsible for signal conversion and termination in purinergic signaling by extracellular nucleotides. Here we present apo and complex structures of the rat NTPDase2 extracellular domain and Legionella pneumophila NTPDase1, including a high-resolution structure with a transition-state analog. Comparison of ATP and ADP binding modes shows how NTPDases engage the same catalytic site for hydrolysis of nucleoside triphosphates and diphosphates. We find that this dual specificity is achieved at the expense of base specificity. Structural and mutational studies indicate that a conserved active-site water is replaced by the phosphate product immediately after phosphoryl transfer. Partial base specificity for purines in LpNTPDase1 is based on a different intersubunit base binding site for pyrimidine bases. A comparison of the bacterial enzyme in six independent crystal forms shows that NTPDases can undergo a domain closure motion of at least 17 degrees .
-==Function==
+Crystallographic Snapshots along the Reaction Pathway of Nucleoside Triphosphate Diphosphohydrolases.,Zebisch M, Krauss M, Schafer P, Lauble P, Strater N Structure. 2013 Jul 2. pii: S0969-2126(13)00200-1. doi:, 10.1016/j.str.2013.05.016. PMID:23830739<ref>PMID:23830739</ref>
-[[http://www.uniprot.org/uniprot/ENTP2_RAT ENTP2_RAT]] In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[4br2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BR2 OCA].
+</div>
-==Reference==
+==See Also==
-<ref group="xtra">PMID:023830739</ref><references group="xtra"/><references/>
+*[[Ectonucleoside triphosphate diphosphohydrolase|Ectonucleoside triphosphate diphosphohydrolase]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Buffalo rat]]
-[[Category: Lauble, P.]]
+[[Category: Lauble, P]]
-[[Category: Schaefer, P.]]
+[[Category: Schaefer, P]]
-[[Category: Straeter, N.]]
+[[Category: Straeter, N]]
-[[Category: Zebisch, M.]]
+[[Category: Zebisch, M]]
 [[Category: Adpase]]
 [[Category: Apyrase]]