1wwr: Difference between revisions
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[[Image:1wwr.gif|left|200px]] | [[Image:1wwr.gif|left|200px]] | ||
'''Crystal structure of tRNA adenosine deaminase TadA from Aquifex aeolicus''' | {{Structure | ||
|PDB= 1wwr |SIZE=350|CAPTION= <scene name='initialview01'>1wwr</scene>, resolution 1.80Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of tRNA adenosine deaminase TadA from Aquifex aeolicus''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1WWR is a [ | 1WWR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WWR OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of tRNA adenosine deaminase (TadA) from Aquifex aeolicus., Kuratani M, Ishii R, Bessho Y, Fukunaga R, Sengoku T, Shirouzu M, Sekine S, Yokoyama S, J Biol Chem. 2005 Apr 22;280(16):16002-8. Epub 2005 Jan 26. PMID:[http:// | Crystal structure of tRNA adenosine deaminase (TadA) from Aquifex aeolicus., Kuratani M, Ishii R, Bessho Y, Fukunaga R, Sengoku T, Shirouzu M, Sekine S, Yokoyama S, J Biol Chem. 2005 Apr 22;280(16):16002-8. Epub 2005 Jan 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15677468 15677468] | ||
[[Category: Aquifex aeolicus]] | [[Category: Aquifex aeolicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: riken structural genomics/proteomics initiative]] | [[Category: riken structural genomics/proteomics initiative]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:02:28 2008'' | ||
Revision as of 16:02, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of tRNA adenosine deaminase TadA from Aquifex aeolicus
OverviewOverview
The bacterial tRNA adenosine deaminase (TadA) generates inosine by deaminating the adenosine residue at the wobble position of tRNA(Arg-2). This modification is essential for the decoding system. In this study, we determined the crystal structure of Aquifex aeolicus TadA at a 1.8-A resolution. This is the first structure of a deaminase acting on tRNA. A. aeolicus TadA has an alpha/beta/alpha three-layered fold and forms a homodimer. The A. aeolicus TadA dimeric structure is completely different from the tetrameric structure of yeast CDD1, which deaminates mRNA and cytidine, but is similar to the dimeric structure of yeast cytosine deaminase. However, in the A. aeolicus TadA structure, the shapes of the C-terminal helix and the regions between the beta4 and beta5 strands are quite distinct from those of yeast cytosine deaminase and a large cavity is produced. This cavity contains many conserved amino acid residues that are likely to be involved in either catalysis or tRNA binding. We made a docking model of TadA with the tRNA anticodon stem loop.
About this StructureAbout this Structure
1WWR is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of tRNA adenosine deaminase (TadA) from Aquifex aeolicus., Kuratani M, Ishii R, Bessho Y, Fukunaga R, Sengoku T, Shirouzu M, Sekine S, Yokoyama S, J Biol Chem. 2005 Apr 22;280(16):16002-8. Epub 2005 Jan 26. PMID:15677468
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