1ww5: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1ww5.gif|left|200px]] | [[Image:1ww5.gif|left|200px]] | ||
'''Agrocybe cylindracea galectin complexed with 3'-sulfonyl lactose''' | {{Structure | ||
|PDB= 1ww5 |SIZE=350|CAPTION= <scene name='initialview01'>1ww5</scene>, resolution 2.20Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Agrocybe cylindracea galectin complexed with 3'-sulfonyl lactose''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1WW5 is a [ | 1WW5 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Agrocybe_cylindracea Agrocybe cylindracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WW5 OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis of a fungal galectin from Agrocybe cylindracea for recognizing sialoconjugate., Ban M, Yoon HJ, Demirkan E, Utsumi S, Mikami B, Yagi F, J Mol Biol. 2005 Aug 26;351(4):695-706. PMID:[http:// | Structural basis of a fungal galectin from Agrocybe cylindracea for recognizing sialoconjugate., Ban M, Yoon HJ, Demirkan E, Utsumi S, Mikami B, Yagi F, J Mol Biol. 2005 Aug 26;351(4):695-706. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16051274 16051274] | ||
[[Category: Agrocybe cylindracea]] | [[Category: Agrocybe cylindracea]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 25: | Line 34: | ||
[[Category: x-ray crystallographic analysis]] | [[Category: x-ray crystallographic analysis]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:02:14 2008'' | ||
Revision as of 16:02, 20 March 2008
| |||||||
| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Agrocybe cylindracea galectin complexed with 3'-sulfonyl lactose
OverviewOverview
Galectin from an edible fungus Agrocybe cylindracea (ACG) has a strong preference for N-acetylneuraminyl lactose (NeuAcalpha2-3lactose). The sugar recognition mechanism of ACG was explored by the X-ray crystallographic analyses of ligand-free ACG, and its complex with lactose, 3'-sulfonyl lactose and NeuAcalpha2-3lactose. The refined structure shows that ACG is a "proto"-type galectin composed of a beta-sandwich of two antiparallel sheets, each with six strands, in contrast to the five and six strands in animal galectins. ACG dimer in solution was classified as being among the "layer"-type. The carbohydrate recognition domain (CRD) of this galectin is common to those of animal galectins, except for substitution of one residue, Ala64, which corresponds to Asn46 in human galectin 1. A five-residue insertion in ACG at positions 42-46 involving Ser44 and Asn46 modified the architecture of the sugar binding site that contributes sialic acid specificity. Furthermore, it was found that the binding of a sulfate ion near the CRD in the ligand-free form led to a change in the conformation of the loop region caused by main-chain cis/trans transition between Ser44 and Pro45.
About this StructureAbout this Structure
1WW5 is a Protein complex structure of sequences from Agrocybe cylindracea. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of a fungal galectin from Agrocybe cylindracea for recognizing sialoconjugate., Ban M, Yoon HJ, Demirkan E, Utsumi S, Mikami B, Yagi F, J Mol Biol. 2005 Aug 26;351(4):695-706. PMID:16051274
Page seeded by OCA on Thu Mar 20 15:02:14 2008