1wmh: Difference between revisions
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[[Image:1wmh.jpg|left|200px]] | [[Image:1wmh.jpg|left|200px]] | ||
'''Crystal structure of a PB1 domain complex of Protein kinase c iota and Par6 alpha''' | {{Structure | ||
|PDB= 1wmh |SIZE=350|CAPTION= <scene name='initialview01'>1wmh</scene>, resolution 1.50Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] | |||
|GENE= PKCiota ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), Par6alpha ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''Crystal structure of a PB1 domain complex of Protein kinase c iota and Par6 alpha''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1WMH is a [ | 1WMH is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WMH OCA]. | ||
==Reference== | ==Reference== | ||
Structure of a cell polarity regulator, a complex between atypical PKC and Par6 PB1 domains., Hirano Y, Yoshinaga S, Takeya R, Suzuki NN, Horiuchi M, Kohjima M, Sumimoto H, Inagaki F, J Biol Chem. 2005 Mar 11;280(10):9653-61. Epub 2004 Dec 7. PMID:[http:// | Structure of a cell polarity regulator, a complex between atypical PKC and Par6 PB1 domains., Hirano Y, Yoshinaga S, Takeya R, Suzuki NN, Horiuchi M, Kohjima M, Sumimoto H, Inagaki F, J Biol Chem. 2005 Mar 11;280(10):9653-61. Epub 2004 Dec 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15590654 15590654] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Non-specific serine/threonine protein kinase]] | [[Category: Non-specific serine/threonine protein kinase]] | ||
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[[Category: pb1 domain]] | [[Category: pb1 domain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:58:54 2008'' | ||
Revision as of 15:58, 20 March 2008
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| , resolution 1.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | PKCiota (Homo sapiens), Par6alpha (Homo sapiens) | ||||||
| Activity: | Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a PB1 domain complex of Protein kinase c iota and Par6 alpha
OverviewOverview
A complex of atypical PKC and Par6 is a common regulator for cell polarity-related processes, which is an essential clue to evolutionary conserved cell polarity regulation. Here, we determined the crystal structure of the complex of PKCiota and Par6alpha PB1 domains to a resolution of 1.5 A. Both PB1 domains adopt a ubiquitin fold. PKCiota PB1 presents an OPR, PC, and AID (OPCA) motif, 28 amino acid residues with acidic and hydrophobic residues, which interacts with the conserved lysine residue of Par6alpha PB1 in a front and back manner. On the interface, several salt bridges are formed including the conserved acidic residues on the OPCA motif of PKCiota PB1 and the conserved lysine residue on the Par6alpha PB1. Structural comparison of the PKCiota and Par6alpha PB1 complex with the p40phox and p67phox PB1 domain complex, subunits of neutrophil NADPH oxidase, reveals that the specific interaction is achieved by tilting the interface so that the insertion or extension in the sequence is engaged in the specificity determinant. The PB1 domain develops the interaction surface on the ubiquitin fold to increase the versatility of molecular interaction.
About this StructureAbout this Structure
1WMH is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Structure of a cell polarity regulator, a complex between atypical PKC and Par6 PB1 domains., Hirano Y, Yoshinaga S, Takeya R, Suzuki NN, Horiuchi M, Kohjima M, Sumimoto H, Inagaki F, J Biol Chem. 2005 Mar 11;280(10):9653-61. Epub 2004 Dec 7. PMID:15590654
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