1wm7: Difference between revisions

Revision as of 15:58, 20 March 2008

Coordinates:

Solution Structure of BmP01 from the Venom of Scorpion Buthus martensii Karsch, 9 structures

OverviewOverview

From the venom of scorpion Buthus martensii Karsch,a short peptide (BmP01, 29 amino acid residues) was isolated and characterized as previously reported (Lebren, R. R., et al. (1997) Eur. J. Biochem. 245, 457-464). It was shown to reduce 33% outward K(+) channel (hippocampal neurons) currents at 10 microM. The solution structure of BmP01 was determined by 2D (1)H NMR spectroscopy. The NOEs, coupling constants, and H-D exchange obtained from NMR spectroscopy were used in structural calculations. The conformation of BmP01 is composed of a short alpha-helix (Cys 3-Thr 12) and a two-stranded antiparallel beta-sheet (Ala 15-Asp 20 and Lys 23-Pro 28). There are three disulfide bridges (Cys 3-Cys 19, Cys 6-Cys 24 and Cys 10-Cys 26) connecting the alpha-helix and beta-sheet. Asp 20 to Lys 23 form a type II turn linking the two strands. Structural and electrostatic potential comparison between BmP01 and its analogues are also presented.

About this StructureAbout this Structure

1WM7 is a Single protein structure of sequence from Mesobuthus martensii. Full crystallographic information is available from OCA.

ReferenceReference

Solution structure of BmP01 from the venom of scorpion Buthus martensii Karsch., Wu G, Li Y, Wei D, He F, Jiang S, Hu G, Wu H, Biochem Biophys Res Commun. 2000 Oct 5;276(3):1148-54. PMID:11027603

Page seeded by OCA on Thu Mar 20 14:58:53 2008

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OCA

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-[[Image:1wm7.jpg|left|200px]]<br /><applet load="1wm7" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1wm7.jpg|left|200px]]
-caption="1wm7" />
-'''Solution Structure of BmP01 from the Venom of Scorpion Buthus martensii Karsch, 9 structures'''<br />
+ {{Structure
+|PDB= 1wm7 |SIZE=350|CAPTION= <scene name='initialview01'>1wm7</scene>
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''Solution Structure of BmP01 from the Venom of Scorpion Buthus martensii Karsch, 9 structures'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-WM7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WM7 OCA].
+WM7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WM7 OCA].
 ==Reference==
-Solution structure of BmP01 from the venom of scorpion Buthus martensii Karsch., Wu G, Li Y, Wei D, He F, Jiang S, Hu G, Wu H, Biochem Biophys Res Commun. 2000 Oct 5;276(3):1148-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11027603 11027603]
+Solution structure of BmP01 from the venom of scorpion Buthus martensii Karsch., Wu G, Li Y, Wei D, He F, Jiang S, Hu G, Wu H, Biochem Biophys Res Commun. 2000 Oct 5;276(3):1148-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11027603 11027603]
 [[Category: Mesobuthus martensii]]
 [[Category: Single protein]]
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 [[Category: alpha/beta scaffold]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:45:52 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:58:53 2008''