1wac: Difference between revisions

Revision as of 15:54, 20 March 2008

, resolution 3.0Å

Coordinates:

BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE

OverviewOverview

The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.

About this StructureAbout this Structure

1WAC is a Single protein structure of sequence from Bacteriophage phi-6. Full crystallographic information is available from OCA.

ReferenceReference

Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:15659773

Page seeded by OCA on Thu Mar 20 14:54:22 2008

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OCA

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-[[Image:1wac.gif|left|200px]]<br /><applet load="1wac" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1wac.gif|left|200px]]
-caption="1wac, resolution 3.0&Aring;" />
-'''BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE'''<br />
+ {{Structure
+|PDB= 1wac |SIZE=350|CAPTION= <scene name='initialview01'>1wac</scene>, resolution 3.0&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE'''
 ==Overview==
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 ==About this Structure==
-WAC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_phi-6 Bacteriophage phi-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA].
+WAC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_phi-6 Bacteriophage phi-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA].
 ==Reference==
-Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15659773 15659773]
+Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15659773 15659773]
 [[Category: Bacteriophage phi-6]]
 [[Category: Single protein]]
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 [[Category: transcription]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:42:08 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:54:22 2008''