1w0u: Difference between revisions

Revision as of 15:50, 20 March 2008

, resolution 1.80Å

Coordinates:

HTRF2 DNA-BINDING DOMAIN IN COMPLEX WITH TELOMERIC DNA.

OverviewOverview

Human telomeres consist of tandem arrays of TTAGGG sequence repeats that are specifically bound by two proteins, TRF1 and TRF2. They bind to DNA as preformed homodimers and have the same architecture in which the DNA-binding domains (Dbds) form independent structural units. Despite these similarities, TRF1 and TRF2 have different functions at telomeres. The X-ray crystal structures of both TRF1- and TRF2-Dbds in complex with telomeric DNA (2.0 and 1.8 angstroms resolution, respectively) show that they recognize the same TAGGGTT binding site by means of homeodomains, as does the yeast telomeric protein Rap1p. Two of the three G-C base pairs that characterize telomeric repeats are recognized specifically and an unusually large number of water molecules mediate protein-DNA interactions. The binding of the TRF2-Dbd to the DNA double helix shows no distortions that would account for the promotion of t-loops in which TRF2 has been implicated.

About this StructureAbout this Structure

1W0U is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

How the human telomeric proteins TRF1 and TRF2 recognize telomeric DNA: a view from high-resolution crystal structures., Court R, Chapman L, Fairall L, Rhodes D, EMBO Rep. 2005 Jan;6(1):39-45. PMID:15608617

Page seeded by OCA on Thu Mar 20 14:50:34 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1w0u.gif|left|200px]]<br /><applet load="1w0u" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1w0u.gif|left|200px]]
-caption="1w0u, resolution 1.80&Aring;" />
-'''HTRF2 DNA-BINDING DOMAIN IN COMPLEX WITH TELOMERIC DNA.'''<br />
+ {{Structure
+|PDB= 1w0u |SIZE=350|CAPTION= <scene name='initialview01'>1w0u</scene>, resolution 1.80&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''HTRF2 DNA-BINDING DOMAIN IN COMPLEX WITH TELOMERIC DNA.'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-W0U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W0U OCA].
+W0U is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W0U OCA].
 ==Reference==
-How the human telomeric proteins TRF1 and TRF2 recognize telomeric DNA: a view from high-resolution crystal structures., Court R, Chapman L, Fairall L, Rhodes D, EMBO Rep. 2005 Jan;6(1):39-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15608617 15608617]
+How the human telomeric proteins TRF1 and TRF2 recognize telomeric DNA: a view from high-resolution crystal structures., Court R, Chapman L, Fairall L, Rhodes D, EMBO Rep. 2005 Jan;6(1):39-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15608617 15608617]
 [[Category: Homo sapiens]]
 [[Category: Protein complex]]
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 [[Category: telomere]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:39:20 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:50:34 2008''