1vsr: Difference between revisions
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[[Image:1vsr.gif|left|200px]] | [[Image:1vsr.gif|left|200px]] | ||
'''VERY SHORT PATCH REPAIR (VSR) ENDONUCLEASE FROM ESCHERICHIA COLI''' | {{Structure | ||
|PDB= 1vsr |SIZE=350|CAPTION= <scene name='initialview01'>1vsr</scene>, resolution 1.8Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |||
|ACTIVITY= | |||
|GENE= VSR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''VERY SHORT PATCH REPAIR (VSR) ENDONUCLEASE FROM ESCHERICHIA COLI''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1VSR is a [ | 1VSR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VSR OCA]. | ||
==Reference== | ==Reference== | ||
Crystallographic and functional studies of very short patch repair endonuclease., Tsutakawa SE, Muto T, Kawate T, Jingami H, Kunishima N, Ariyoshi M, Kohda D, Nakagawa M, Morikawa K, Mol Cell. 1999 May;3(5):621-8. PMID:[http:// | Crystallographic and functional studies of very short patch repair endonuclease., Tsutakawa SE, Muto T, Kawate T, Jingami H, Kunishima N, Ariyoshi M, Kohda D, Nakagawa M, Morikawa K, Mol Cell. 1999 May;3(5):621-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10360178 10360178] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: mismatch recognition]] | [[Category: mismatch recognition]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:49:15 2008'' | ||
Revision as of 15:49, 20 March 2008
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| , resolution 1.8Å | |||||||
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| Ligands: | |||||||
| Gene: | VSR (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
VERY SHORT PATCH REPAIR (VSR) ENDONUCLEASE FROM ESCHERICHIA COLI
OverviewOverview
Vsr endonuclease plays a crucial role in the repair of TG mismatched base pairs, which are generated by the spontaneous degradation of methylated cytidines; Vsr recognizes the mismatched base pair and cleaves the phosphate backbone 5' to the thymidine. We have determined the crystal structure of a truncated form of this endonuclease at 1.8 A resolution. The protein contains one structural zinc-binding module. Unexpectedly, its overall topology resembles members of the type II restriction endonuclease family. Subsequent mutational and biochemical analyses showed that certain elements in the catalytic site are also conserved. However, the identification of a critical histidine and evidence of an active site metal-binding coordination that is novel to endonucleases indicate a distinct catalytic mechanism.
About this StructureAbout this Structure
1VSR is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Crystallographic and functional studies of very short patch repair endonuclease., Tsutakawa SE, Muto T, Kawate T, Jingami H, Kunishima N, Ariyoshi M, Kohda D, Nakagawa M, Morikawa K, Mol Cell. 1999 May;3(5):621-8. PMID:10360178
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