1vqe: Difference between revisions

Revision as of 15:48, 20 March 2008

, resolution 1.8Å

Coordinates:

GENE V PROTEIN MUTANT WITH VAL 35 REPLACED BY ILE 35 AND ILE 47 REPLACED BY MET 47 (V35I, I47M)

OverviewOverview

The problem of rationally engineering protein molecules can be simplified where effects of mutations on protein function are additive. Crystal structures of single and double mutants in the hydrophobic core of gene V protein indicate that structural and functional effects of core mutations are additive when the regions structurally influenced by the mutations do not substantially overlap. These regions of influence can provide a simple basis for identifying sets of mutations that will show additive effects.

About this StructureAbout this Structure

1VQE is a Single protein structure of sequence from Bacteriophage f1. Full crystallographic information is available from OCA.

ReferenceReference

Potential use of additivity of mutational effects in simplifying protein engineering., Skinner MM, Terwilliger TC, Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10753-7. PMID:8855252

Page seeded by OCA on Thu Mar 20 14:48:24 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1vqe.jpg|left|200px]]<br /><applet load="1vqe" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1vqe.jpg|left|200px]]
-caption="1vqe, resolution 1.8&Aring;" />
-'''GENE V PROTEIN MUTANT WITH VAL 35 REPLACED BY ILE 35 AND ILE 47 REPLACED BY MET 47 (V35I, I47M)'''<br />
+ {{Structure
+|PDB= 1vqe |SIZE=350|CAPTION= <scene name='initialview01'>1vqe</scene>, resolution 1.8&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''GENE V PROTEIN MUTANT WITH VAL 35 REPLACED BY ILE 35 AND ILE 47 REPLACED BY MET 47 (V35I, I47M)'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-VQE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_f1 Bacteriophage f1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VQE OCA].
+VQE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_f1 Bacteriophage f1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VQE OCA].
 ==Reference==
-Potential use of additivity of mutational effects in simplifying protein engineering., Skinner MM, Terwilliger TC, Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10753-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8855252 8855252]
+Potential use of additivity of mutational effects in simplifying protein engineering., Skinner MM, Terwilliger TC, Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10753-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8855252 8855252]
 [[Category: Bacteriophage f1]]
 [[Category: Single protein]]
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 [[Category: mutant]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:37:31 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:48:24 2008''