Endonuclease: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older editNewer edit →
Michal Harel (talk | contribs)
31,761 edits
No edit summary
Michal Harel (talk | contribs)
31,761 edits
No edit summary
Line 5: Line 5:
to a Jmol applet. Check out the other buttons as well!  
to a Jmol applet. Check out the other buttons as well!  
-->
-->
'''Endonuclease''' (ENN) cleaves phosphodiester bond within polynucleotide chain.  ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome.  ENN is restriction site–specific.  Various types of ENN differ by their mechanism of action.  ENN is used in genetic engineering to make recombinant DNA.  ENN requires a restriction site and a cleavage pattern.  ENN-I operates on DNA with separate restriction site and cleavage pattern, while ENN-II operates on overlapping restriction site and cleavage pattern.  Some ENNs are encoded within introns thus facilitating their mobility.  These ENNs or inteins are designated I-ENN. The '''Cas ENN''' proteins are part of CRISPR/Cas prokaryotic immune system which confers protection from foreign genetic elements.  The CRISPR (Custered Regularly Interspersed Short Palindromic Repeats) are DNA loci which are found in ca. 40% of the bacteria.  For more details see [[Cas9]]. See also<br />
'''Endonuclease''' (ENN) cleaves phosphodiester bond within polynucleotide chain.  ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome.  ENN is restriction site–specific.  Various types of ENN differ by their mechanism of action.  ENN is used in genetic engineering to make recombinant DNA.  ENN requires a restriction site and a cleavage pattern.  ENN-I operates on DNA with separate restriction site and cleavage pattern, while ENN-II operates on overlapping restriction site and cleavage pattern.  Some ENNs are encoded within introns thus facilitating their mobility.  These ENNs or inteins are designated I-ENN.<br />
The '''Cas ENN''' proteins are part of CRISPR/Cas prokaryotic immune system which confers protection from foreign genetic elements.  The CRISPR (Custered Regularly Interspersed Short Palindromic Repeats) are DNA loci which are found in ca. 40% of the bacteria.  The CRISPR/Cas system is being used lately as gene editing tool.  For more details see [[Cas9]].<br /> See also<br />
* [[Apurinic-Apyrimidinic Endonuclease-1]]<br />
* [[Apurinic-Apyrimidinic Endonuclease-1]]<br />
* [[DNA repair protein human endonuclease VIII-like 1 (NEIL1)]] .
* [[DNA repair protein human endonuclease VIII-like 1 (NEIL1)]] .

Revision as of 13:54, 23 December 2014

Template:STRUCTURE 1rva Endonuclease (ENN) cleaves phosphodiester bond within polynucleotide chain. ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome. ENN is restriction site–specific. Various types of ENN differ by their mechanism of action. ENN is used in genetic engineering to make recombinant DNA. ENN requires a restriction site and a cleavage pattern. ENN-I operates on DNA with separate restriction site and cleavage pattern, while ENN-II operates on overlapping restriction site and cleavage pattern. Some ENNs are encoded within introns thus facilitating their mobility. These ENNs or inteins are designated I-ENN.
The Cas ENN proteins are part of CRISPR/Cas prokaryotic immune system which confers protection from foreign genetic elements. The CRISPR (Custered Regularly Interspersed Short Palindromic Repeats) are DNA loci which are found in ca. 40% of the bacteria. The CRISPR/Cas system is being used lately as gene editing tool. For more details see Cas9.
See also

3D structures of endonuclease3D structures of endonuclease

Updated on 23-December-2014

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=Endonuclease&oldid=2182088"