1vfz: Difference between revisions

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Revision as of 15:45, 20 March 2008

File:1vfz.gif

, resolution 2.24Å

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Crystal Structure of the Kif1A Motor Domain Complexed With ADP-Mg-VO4

OverviewOverview

The motor protein kinesin moves along microtubules, driven by adenosine triphosphate (ATP) hydrolysis. However, it remains unclear how kinesin converts the chemical energy into mechanical movement. We report crystal structures of monomeric kinesin KIF1A with three transition-state analogs: adenylyl imidodiphosphate (AMP-PNP), adenosine diphosphate (ADP)-vanadate, and ADP-AlFx (aluminofluoride complexes). These structures, together with known structures of the ADP-bound state and the adenylyl-(beta,gamma-methylene) diphosphate (AMP-PCP)-bound state, show that kinesin uses two microtubule-binding loops in an alternating manner to change its interaction with microtubules during the ATP hydrolysis cycle; loop L11 is extended in the AMP-PNP structure, whereas loop L12 is extended in the ADP structure. ADP-vanadate displays an intermediate structure in which a conformational change in two switch regions causes both loops to be raised from the microtubule, thus actively detaching kinesin.

About this StructureAbout this Structure

1VFZ is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

KIF1A alternately uses two loops to bind microtubules., Nitta R, Kikkawa M, Okada Y, Hirokawa N, Science. 2004 Jul 30;305(5684):678-83. PMID:15286375

Page seeded by OCA on Thu Mar 20 14:45:01 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1vfz.gif|left|200px]]<br /><applet load="1vfz" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1vfz.gif|left|200px]]
-caption="1vfz, resolution 2.24&Aring;" />
-'''Crystal Structure of the Kif1A Motor Domain Complexed With ADP-Mg-VO4'''<br />
+ {{Structure
+|PDB= 1vfz |SIZE=350|CAPTION= <scene name='initialview01'>1vfz</scene>, resolution 2.24&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Crystal Structure of the Kif1A Motor Domain Complexed With ADP-Mg-VO4'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-VFZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=VO4:'>VO4</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VFZ OCA].
+VFZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VFZ OCA].
 ==Reference==
-KIF1A alternately uses two loops to bind microtubules., Nitta R, Kikkawa M, Okada Y, Hirokawa N, Science. 2004 Jul 30;305(5684):678-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15286375 15286375]
+KIF1A alternately uses two loops to bind microtubules., Nitta R, Kikkawa M, Okada Y, Hirokawa N, Science. 2004 Jul 30;305(5684):678-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15286375 15286375]
 [[Category: Mus musculus]]
 [[Category: Single protein]]
@@ Line 24: / Line 33: @@
 [[Category: motor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:35:00 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:45:01 2008''