1e8j: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1e8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_gigas Desulfovibrio gigas]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E8J FirstGlance]. <br> | <table><tr><td colspan='2'>[[1e8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_gigas Desulfovibrio gigas]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E8J FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e8j RCSB], [http://www.ebi.ac.uk/pdbsum/1e8j PDBsum]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e8j RCSB], [http://www.ebi.ac.uk/pdbsum/1e8j PDBsum]</span></td></tr> | ||
<table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Desulfovibrio gigas]] | [[Category: Desulfovibrio gigas]] | ||
[[Category: Brennan, L | [[Category: Brennan, L]] | ||
[[Category: Lamosa, P | [[Category: Lamosa, P]] | ||
[[Category: Santos, H | [[Category: Santos, H]] | ||
[[Category: Turner, D L | [[Category: Turner, D L]] | ||
[[Category: Vis, H | [[Category: Vis, H]] | ||
[[Category: Electron transport]] | [[Category: Electron transport]] | ||
[[Category: Thermostability]] | [[Category: Thermostability]] | ||
[[Category: Zinc-substitution]] | [[Category: Zinc-substitution]] | ||
Revision as of 01:44, 23 December 2014
SOLUTION STRUCTURE OF DESULFOVIBRIO GIGAS ZINC RUBREDOXIN, NMR, 20 STRUCTURESSOLUTION STRUCTURE OF DESULFOVIBRIO GIGAS ZINC RUBREDOXIN, NMR, 20 STRUCTURES
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedRubredoxins are small, soluble proteins that display a wide variation in thermostability, despite having a high degree of sequence similarity They also vary in the extent to which they are stabilized by solutes such as diglycerol phosphate. Hence, they provide excellent models for studying the mechanisms of thermostabilization. Nuclear magnetic resonance (NMR) spectroscopy can be used to investigate interactions between molecules, as well as subtle changes in conformation in solution, and also provides a means to measure protein stability. The assignment of the proton NMR spectrum of the zinc rubredoxin from Desulfovibrio gigas is presented, together with its structure in solution. The stabilizing effect of diglycerol phosphate on rubredoxin is demonstrated and assessed by determining selected amide proton exchange rates; diglycerol phosphate at 100 mM concentration caused an additional structural stabilization of 1.2 +/-0.4 kJ/mol. The pattern of effects on the exchange rates is discussed in relation to the protein structure. NMR structure of Desulfovibrio gigas rubredoxin: a model for studying protein stabilization by compatible solutes.,Lamosa P, Brennan L, Vis H, Turner DL, Santos H Extremophiles. 2001 Oct;5(5):303-11. PMID:11699644[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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