1v03: Difference between revisions
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[[Image:1v03.gif|left|200px]] | [[Image:1v03.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF THE SORGHUM BICOLOR DHURRINASE 1''' | {{Structure | ||
|PDB= 1v03 |SIZE=350|CAPTION= <scene name='initialview01'>1v03</scene>, resolution 2.00Å | |||
|SITE= <scene name='pdbsite=AC1:Iph+Binding+Site+For+Chain+A'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene> and <scene name='pdbligand=CCN:ACETONITRILE'>CCN</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF THE SORGHUM BICOLOR DHURRINASE 1''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1V03 is a [ | 1V03 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sorghum_bicolor Sorghum bicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V03 OCA]. | ||
==Reference== | ==Reference== | ||
Structural determinants of substrate specificity in family 1 beta-glucosidases: novel insights from the crystal structure of sorghum dhurrinase-1, a plant beta-glucosidase with strict specificity, in complex with its natural substrate., Verdoucq L, Moriniere J, Bevan DR, Esen A, Vasella A, Henrissat B, Czjze M, J Biol Chem. 2004 Jul 23;279(30):31796-803. Epub 2004 May 17. PMID:[http:// | Structural determinants of substrate specificity in family 1 beta-glucosidases: novel insights from the crystal structure of sorghum dhurrinase-1, a plant beta-glucosidase with strict specificity, in complex with its natural substrate., Verdoucq L, Moriniere J, Bevan DR, Esen A, Vasella A, Henrissat B, Czjze M, J Biol Chem. 2004 Jul 23;279(30):31796-803. Epub 2004 May 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15148317 15148317] | ||
[[Category: Beta-glucosidase]] | [[Category: Beta-glucosidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: pest defense]] | [[Category: pest defense]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:39:08 2008'' | ||
Revision as of 15:39, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Sites: | |||||||
| Ligands: | , and | ||||||
| Activity: | Beta-glucosidase, with EC number 3.2.1.21 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE SORGHUM BICOLOR DHURRINASE 1
OverviewOverview
Plant beta-glucosidases play a crucial role in defense against pests. They cleave, with variable specificity, beta-glucosides to release toxic aglycone moieties. The Sorghum bicolor beta-glucosidase isoenzyme Dhr1 has a strict specificity for its natural substrate dhurrin (p-hydroxy-(S)-mandelonitrile-beta-D-glucoside), whereas its close homolog, the maize beta-glucosidase isoenzyme Glu1, which shares 72% sequence identity, hydrolyzes a broad spectrum of substrates in addition to its natural substrate 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxaxin-3-one. Structural data from enzyme.substrate complexes of Dhr1 show that the mode of aglycone binding differs from that previously observed in the homologous maize enzyme. Specifically, the data suggest that Asn(259), Phe(261), and Ser(462), located in the aglycone-binding site of S. bicolor Dhr1, are crucial for aglycone recognition and binding. The tight binding of the aglycone moiety of dhurrin promotes the stabilization of the reaction intermediate in which the glycone moiety is in a deformed (1)S(3) conformation within the glycone-binding site, ready for nucleophilic attack to occur. Compared with the broad specificity maize beta-glucosidase, this different binding mode explains the narrow specificity of sorghum dhurrinase-1.
About this StructureAbout this Structure
1V03 is a Single protein structure of sequence from Sorghum bicolor. Full crystallographic information is available from OCA.
ReferenceReference
Structural determinants of substrate specificity in family 1 beta-glucosidases: novel insights from the crystal structure of sorghum dhurrinase-1, a plant beta-glucosidase with strict specificity, in complex with its natural substrate., Verdoucq L, Moriniere J, Bevan DR, Esen A, Vasella A, Henrissat B, Czjze M, J Biol Chem. 2004 Jul 23;279(30):31796-803. Epub 2004 May 17. PMID:15148317
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