1uwp: Difference between revisions
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[[Image:1uwp.gif|left|200px]] | [[Image:1uwp.gif|left|200px]] | ||
'''INITIAL EVENTS IN THE PHOTOCYCLE OF PHOTOACTIVE YELLOW PROTEIN''' | {{Structure | ||
|PDB= 1uwp |SIZE=350|CAPTION= <scene name='initialview01'>1uwp</scene>, resolution 1.20Å | |||
|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+X'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=HC4:4'-HYDROXYCINNAMIC ACID'>HC4</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''INITIAL EVENTS IN THE PHOTOCYCLE OF PHOTOACTIVE YELLOW PROTEIN''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1UWP is a [ | 1UWP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UWP OCA]. | ||
==Reference== | ==Reference== | ||
Initial events in the photocycle of photoactive yellow protein., Kort R, Hellingwerf KJ, Ravelli RB, J Biol Chem. 2004 Jun 18;279(25):26417-24. Epub 2004 Mar 16. PMID:[http:// | Initial events in the photocycle of photoactive yellow protein., Kort R, Hellingwerf KJ, Ravelli RB, J Biol Chem. 2004 Jun 18;279(25):26417-24. Epub 2004 Mar 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15026418 15026418] | ||
[[Category: Halorhodospira halophila]] | [[Category: Halorhodospira halophila]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: HC4]] | [[Category: HC4]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: | [[Category: domain]] | ||
[[Category: fold photocycle]] | [[Category: fold photocycle]] | ||
[[Category: gaf]] | [[Category: gaf]] | ||
[[Category: lov]] | [[Category: lov]] | ||
[[Category: | [[Category: pa]] | ||
[[Category: photoactive]] | [[Category: photoactive]] | ||
[[Category: photoreceptor]] | [[Category: photoreceptor]] | ||
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[[Category: yellow]] | [[Category: yellow]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:37:50 2008'' | ||
Revision as of 15:37, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
INITIAL EVENTS IN THE PHOTOCYCLE OF PHOTOACTIVE YELLOW PROTEIN
OverviewOverview
The light-induced isomerization of a double bond is the key event that allows the conversion of light energy into a structural change in photoactive proteins for many light-mediated biological processes, such as vision, photosynthesis, photomorphogenesis, and photo movement. Cofactors such as retinals, linear tetrapyrroles, and 4-hydroxy-cinnamic acid have been selected by nature that provide the essential double bond to transduce the light signal into a conformational change and eventually, a physiological response. Here we report the first events after light excitation of the latter chromophore, containing a single ethylene double bond, in a low temperature crystallographic study of the photoactive yellow protein. We measured experimental phases to overcome possible model bias, corrected for minimized radiation damage, and measured absorption spectra of crystals to analyze the photoproducts formed. The data show a mechanism for the light activation of photoactive yellow protein, where the energy to drive the remainder of the conformational changes is stored in a slightly strained but fully cis-chromophore configuration. In addition, our data indicate a role for backbone rearrangements during the very early structural events.
About this StructureAbout this Structure
1UWP is a Single protein structure of sequence from Halorhodospira halophila. Full crystallographic information is available from OCA.
ReferenceReference
Initial events in the photocycle of photoactive yellow protein., Kort R, Hellingwerf KJ, Ravelli RB, J Biol Chem. 2004 Jun 18;279(25):26417-24. Epub 2004 Mar 16. PMID:15026418
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