1uw4: Difference between revisions

Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism by which, eukaryotic cells detect and degrade transcripts containing premature, termination codons. Three 'up-frameshift' proteins, UPF1, UPF2 and UPF3, are essential for this process in organisms ranging from yeast to human., We present a crystal structure at a resolution of 1.95 A of the complex, between the interacting domains of human UPF2 and UPF3b, which are, respectively, a MIF4G (middle portion of eIF4G) domain and an RNP domain, (ribonucleoprotein-type RNA-binding domain). The protein-protein interface, is mediated by highly conserved charged residues in UPF2 and UPF3b and, involves the beta-sheet surface of the UPF3b RNP domain, which is, generally used by these domains to bind nucleic acids. We show that the, UPF3b RNP does not bind RNA, whereas the UPF2 construct and the complex, do. Our results advance understanding of the molecular mechanisms, underlying the NMD quality control process.

1UW4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with BME as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UW4 OCA].  

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 12:55:04 2007''