1uhm: Difference between revisions
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[[Image:1uhm.gif|left|200px]] | [[Image:1uhm.gif|left|200px]] | ||
'''Solution structure of the globular domain of linker histone homolog Hho1p from S. cerevisiae''' | {{Structure | ||
|PDB= 1uhm |SIZE=350|CAPTION= <scene name='initialview01'>1uhm</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= HHO1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |||
}} | |||
'''Solution structure of the globular domain of linker histone homolog Hho1p from S. cerevisiae''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1UHM is a [ | 1UHM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHM OCA]. | ||
==Reference== | ==Reference== | ||
The linker histone homolog Hho1p from Saccharomyces cerevisiae represents a winged helix-turn-helix fold as determined by NMR spectroscopy., Ono K, Kusano O, Shimotakahara S, Shimizu M, Yamazaki T, Shindo H, Nucleic Acids Res. 2003 Dec 15;31(24):7199-207. PMID:[http:// | The linker histone homolog Hho1p from Saccharomyces cerevisiae represents a winged helix-turn-helix fold as determined by NMR spectroscopy., Ono K, Kusano O, Shimotakahara S, Shimizu M, Yamazaki T, Shindo H, Nucleic Acids Res. 2003 Dec 15;31(24):7199-207. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14654695 14654695] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: rsgi]] | [[Category: rsgi]] | ||
[[Category: s. cerevisiae]] | [[Category: s. cerevisiae]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
[[Category: winged helix-turn-helix]] | [[Category: winged helix-turn-helix]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:32:06 2008'' | ||
Revision as of 15:32, 20 March 2008
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| Gene: | HHO1 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of the globular domain of linker histone homolog Hho1p from S. cerevisiae
OverviewOverview
Hho1p is assumed to serve as a linker histone in Saccharomyces cerevisiae and, notably, it possesses two putative globular domains, designated HD1 (residues 41-118) and HD2 (residues 171-252), that are homologous to histone H5 from chicken erythrocytes. We have determined the three-dimensional structure of globular domain HD1 with high precision by heteronuclear magnetic resonance spectroscopy. The structure had a winged helix-turn-helix motif composed of an alphabetaalphaalphabetabeta fold and closely resembled the structure of the globular domain of histone H5. Interestingly, the second globular domain, HD2, in Hho1p was unstructured under physiological conditions. Gel mobility assay demonstrated that Hho1p preferentially binds to supercoiled DNA over linearized DNA. Furthermore, NMR analysis of the complex of a deletion mutant protein (residues 1-118) of Hho1p with a linear DNA duplex revealed that four regions within the globular domain HD1 are involved in the DNA binding. The above results suggested that Hho1p possesses properties similar to those of linker histones in higher eukaryotes in terms of the structure and binding preference towards supercoiled DNA.
About this StructureAbout this Structure
1UHM is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
The linker histone homolog Hho1p from Saccharomyces cerevisiae represents a winged helix-turn-helix fold as determined by NMR spectroscopy., Ono K, Kusano O, Shimotakahara S, Shimizu M, Yamazaki T, Shindo H, Nucleic Acids Res. 2003 Dec 15;31(24):7199-207. PMID:14654695
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Saccharomyces cerevisiae
- Single protein
- Kusano, O.
- Ono, K.
- RSGI, RIKEN Structural Genomics/Proteomics Initiative.
- Shimizu, M.
- Shimotakahara, S.
- Shindo, H.
- Yamazaki, T.
- Linker histone
- Riken structural genomics/proteomics initiative
- Rsgi
- S. cerevisiae
- Structural genomic
- Winged helix-turn-helix