1ue5: Difference between revisions

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, resolution 2.60Å

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Crystal structure of the single-stranded dna-binding protein from mycobacterium tuberculosis

OverviewOverview

Single-stranded DNA-binding protein (SSB) is an essential protein necessary for the functioning of the DNA replication, repair and recombination machineries. Here we report the structure of the DNA-binding domain of Mycobacterium tuberculosis SSB (MtuSSB) in four different crystals distributed in two forms. The structure of one of the forms was solved by a combination of isomorphous replacement and anomalous scattering. This structure was used to determine the structure of the other form by molecular replacement. The polypeptide chain in the structure exhibits the oligonucleotide binding fold. The globular core of the molecule in different subunits in the two forms and those in Escherichia coli SSB (EcoSSB) and human mitochondrial SSB (HMtSSB) have similar structure, although the three loops exhibit considerable structural variation. However, the tetrameric MtuSSB has an as yet unobserved quaternary association. This quaternary structure with a unique dimeric interface lends the oligomeric protein greater stability, which may be of significance to the functioning of the protein under conditions of stress. Also, as a result of the variation in the quaternary structure the path adopted by the DNA to wrap around MtuSSB is expected to be different from that of EcoSSB.

About this StructureAbout this Structure

1UE5 is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

Structure of Mycobacterium tuberculosis single-stranded DNA-binding protein. Variability in quaternary structure and its implications., Saikrishnan K, Jeyakanthan J, Venkatesh J, Acharya N, Sekar K, Varshney U, Vijayan M, J Mol Biol. 2003 Aug 8;331(2):385-93. PMID:12888346

Page seeded by OCA on Thu Mar 20 14:30:50 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1ue5.gif|left|200px]]<br /><applet load="1ue5" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ue5.gif|left|200px]]
-caption="1ue5, resolution 2.60&Aring;" />
-'''Crystal structure of the single-stranded dna-binding protein from mycobacterium tuberculosis'''<br />
+ {{Structure
+|PDB= 1ue5 |SIZE=350|CAPTION= <scene name='initialview01'>1ue5</scene>, resolution 2.60&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=CD:CADMIUM ION'>CD</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Crystal structure of the single-stranded dna-binding protein from mycobacterium tuberculosis'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-UE5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UE5 OCA].
+UE5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UE5 OCA].
 ==Reference==
-Structure of Mycobacterium tuberculosis single-stranded DNA-binding protein. Variability in quaternary structure and its implications., Saikrishnan K, Jeyakanthan J, Venkatesh J, Acharya N, Sekar K, Varshney U, Vijayan M, J Mol Biol. 2003 Aug 8;331(2):385-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12888346 12888346]
+Structure of Mycobacterium tuberculosis single-stranded DNA-binding protein. Variability in quaternary structure and its implications., Saikrishnan K, Jeyakanthan J, Venkatesh J, Acharya N, Sekar K, Varshney U, Vijayan M, J Mol Biol. 2003 Aug 8;331(2):385-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12888346 12888346]
 [[Category: Mycobacterium tuberculosis]]
 [[Category: Single protein]]
@@ Line 26: / Line 35: @@
 [[Category: protein structure initiative]]
 [[Category: psi]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
 [[Category: tb structural genomics consortium]]
 [[Category: tbsgc]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:23:34 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:30:50 2008''