1a64: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1a64]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A64 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A64 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1a64]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A64 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A64 FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a64 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a64 RCSB], [http://www.ebi.ac.uk/pdbsum/1a64 PDBsum]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a64 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a64 RCSB], [http://www.ebi.ac.uk/pdbsum/1a64 PDBsum]</span></td></tr> | ||
<table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Barker, J J | [[Category: Barker, J J]] | ||
[[Category: Brady, R L | [[Category: Brady, R L]] | ||
[[Category: Head, J G | [[Category: Head, J G]] | ||
[[Category: Murray, A J | [[Category: Murray, A J]] | ||
[[Category: Domain swapping]] | [[Category: Domain swapping]] | ||
[[Category: Hinge loop]] | [[Category: Hinge loop]] | ||
[[Category: Oligomer evolution]] | [[Category: Oligomer evolution]] | ||
Revision as of 14:26, 22 December 2014
ENGINEERING A MISFOLDED FORM OF RAT CD2ENGINEERING A MISFOLDED FORM OF RAT CD2
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe amino-terminal domain of CD2 has the remarkable ability to fold in two ways: either as a monomer or as an intertwined, metastable dimer. Here we show that it is possible to differentially stabilize either fold by engineering the CD2 sequence, mimicking random mutagenesis events that could occur during molecular evolution. Crystal structures of a hinge-deletion mutant, which is stable as an intertwined dimer, reveal domain rotations that enable the protein to further assemble to a tetramer. These results demonstrate that a variety of folds can be adopted by a single polypeptide sequence, and provide guidance for the design of proteins capable of further assembly. Engineering an intertwined form of CD2 for stability and assembly.,Murray AJ, Head JG, Barker JJ, Brady RL Nat Struct Biol. 1998 Sep;5(9):778-82. PMID:9731771[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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