1u3o: Difference between revisions
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[[Image:1u3o.gif|left|200px]] | [[Image:1u3o.gif|left|200px]] | ||
'''Solution structure of rat Kalirin N-terminal SH3 domain''' | {{Structure | ||
|PDB= 1u3o |SIZE=350|CAPTION= <scene name='initialview01'>1u3o</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= Kalirin, HAPIP, Duo ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | |||
}} | |||
'''Solution structure of rat Kalirin N-terminal SH3 domain''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1U3O is a [ | 1U3O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U3O OCA]. | ||
==Reference== | ==Reference== | ||
Regulation of RhoGEF activity by intramolecular and intermolecular SH3 domain interactions., Schiller MR, Chakrabarti K, King GF, Schiller NI, Eipper BA, Maciejewski MW, J Biol Chem. 2006 Jul 7;281(27):18774-86. Epub 2006 Apr 27. PMID:[http:// | Regulation of RhoGEF activity by intramolecular and intermolecular SH3 domain interactions., Schiller MR, Chakrabarti K, King GF, Schiller NI, Eipper BA, Maciejewski MW, J Biol Chem. 2006 Jul 7;281(27):18774-86. Epub 2006 Apr 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16644733 16644733] | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sh3]] | [[Category: sh3]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:26:58 2008'' | ||
Revision as of 15:27, 20 March 2008
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| Gene: | Kalirin, HAPIP, Duo (Rattus norvegicus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of rat Kalirin N-terminal SH3 domain
OverviewOverview
RhoGEFs are central controllers of small G-proteins in cells and are regulated by several mechanisms. There are at least 22 human RhoGEFs that contain SH3 domains, raising the possibility that, like several other enzymes, SH3 domains control the enzymatic activity of guanine nucleotide exchange factor (GEF) domains through intra- and/or intermolecular interactions. The structure of the N-terminal SH3 domain of Kalirin was solved using NMR spectroscopy, and it folds much like other SH3 domains. However, NMR chemical shift mapping experiments showed that this Kalirin SH3 domain is unique, containing novel cooperative binding site(s) for intramolecular PXXP ligands. Intramolecular Kalirin SH3 domain/ligand interactions, as well as binding of the Kalirin SH3 domain to the adaptor protein Crk, inhibit the GEF activity of Kalirin. This study establishes a novel molecular mechanism whereby intramolecular and intermolecular Kalirin SH3 domain/ligand interactions modulate GEF activity, a regulatory mechanism that is likely used by other RhoGEF family members.
About this StructureAbout this Structure
1U3O is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
ReferenceReference
Regulation of RhoGEF activity by intramolecular and intermolecular SH3 domain interactions., Schiller MR, Chakrabarti K, King GF, Schiller NI, Eipper BA, Maciejewski MW, J Biol Chem. 2006 Jul 7;281(27):18774-86. Epub 2006 Apr 27. PMID:16644733
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