1u0d: Difference between revisions
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'''Y33H Mutatant of Homing endonuclease I-CreI''' | {{Structure | ||
|PDB= 1u0d |SIZE=350|CAPTION= <scene name='initialview01'>1u0d</scene>, resolution 2.9Å | |||
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'''Y33H Mutatant of Homing endonuclease I-CreI''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1U0D is a [ | 1U0D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0D OCA]. | ||
==Reference== | ==Reference== | ||
Isolation and characterization of new homing endonuclease specificities at individual target site positions., Sussman D, Chadsey M, Fauce S, Engel A, Bruett A, Monnat R Jr, Stoddard BL, Seligman LM, J Mol Biol. 2004 Sep 3;342(1):31-41. PMID:[http:// | Isolation and characterization of new homing endonuclease specificities at individual target site positions., Sussman D, Chadsey M, Fauce S, Engel A, Bruett A, Monnat R Jr, Stoddard BL, Seligman LM, J Mol Biol. 2004 Sep 3;342(1):31-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15313605 15313605] | ||
[[Category: Chlamydomonas reinhardtii]] | [[Category: Chlamydomonas reinhardtii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein/dna]] | [[Category: protein/dna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:25:36 2008'' | ||
Revision as of 15:25, 20 March 2008
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Y33H Mutatant of Homing endonuclease I-CreI
OverviewOverview
Homing endonucleases are highly specific DNA endonucleases, encoded within mobile introns or inteins, that induce targeted recombination, double-strand repair and gene conversion of their cognate target sites. Due to their biological function and high level of target specificity, these enzymes are under intense investigation as tools for gene targeting. These studies require that naturally occurring enzymes be redesigned to recognize novel target sites. Here, we report studies in which the homodimeric LAGLIDADG homing endonuclease I-CreI is altered at individual side-chains corresponding to contact points to distinct base-pairs in its target site. The resulting enzyme constructs drive specific elimination of selected DNA targets in vivo and display shifted specificities of DNA binding and cleavage in vitro. Crystal structures of two of these constructs demonstrate that substitution of individual side-chain/DNA contact patterns can occur with almost no structural deformation or rearrangement of the surrounding complex, facilitating an isolated, modular redesign strategy for homing endonuclease activity and specificity.
About this StructureAbout this Structure
1U0D is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.
ReferenceReference
Isolation and characterization of new homing endonuclease specificities at individual target site positions., Sussman D, Chadsey M, Fauce S, Engel A, Bruett A, Monnat R Jr, Stoddard BL, Seligman LM, J Mol Biol. 2004 Sep 3;342(1):31-41. PMID:15313605
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