1u04: Difference between revisions
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[[Image:1u04.gif|left|200px]] | [[Image:1u04.gif|left|200px]] | ||
'''Crystal structure of full length Argonaute from Pyrococcus furiosus''' | {{Structure | ||
|PDB= 1u04 |SIZE=350|CAPTION= <scene name='initialview01'>1u04</scene>, resolution 2.25Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of full length Argonaute from Pyrococcus furiosus''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1U04 is a [ | 1U04 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U04 OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of Argonaute and its implications for RISC slicer activity., Song JJ, Smith SK, Hannon GJ, Joshua-Tor L, Science. 2004 Sep 3;305(5689):1434-7. Epub 2004 Jul 29. PMID:[http:// | Crystal structure of Argonaute and its implications for RISC slicer activity., Song JJ, Smith SK, Hannon GJ, Joshua-Tor L, Science. 2004 Sep 3;305(5689):1434-7. Epub 2004 Jul 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15284453 15284453] | ||
[[Category: Pyrococcus furiosus dsm 3638]] | [[Category: Pyrococcus furiosus dsm 3638]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: slicer]] | [[Category: slicer]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:25:32 2008'' | ||
Revision as of 15:25, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of full length Argonaute from Pyrococcus furiosus
OverviewOverview
Argonaute proteins and small interfering RNAs (siRNAs) are the known signature components of the RNA interference effector complex RNA-induced silencing complex (RISC). However, the identity of "Slicer," the enzyme that cleaves the messenger RNA (mRNA) as directed by the siRNA, has not been resolved. Here, we report the crystal structure of the Argonaute protein from Pyrococcus furiosus at 2.25 angstrom resolution. The structure reveals a crescent-shaped base made up of the amino-terminal, middle, and PIWI domains. The Piwi Argonaute Zwille (PAZ) domain is held above the base by a "stalk"-like region. The PIWI domain (named for the protein piwi) is similar to ribonuclease H, with a conserved active site aspartate-aspartate-glutamate motif, strongly implicating Argonaute as "Slicer." The architecture of the molecule and the placement of the PAZ and PIWI domains define a groove for substrate binding and suggest a mechanism for siRNA-guided mRNA cleavage.
About this StructureAbout this Structure
1U04 is a Single protein structure of sequence from Pyrococcus furiosus dsm 3638. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of Argonaute and its implications for RISC slicer activity., Song JJ, Smith SK, Hannon GJ, Joshua-Tor L, Science. 2004 Sep 3;305(5689):1434-7. Epub 2004 Jul 29. PMID:15284453
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