1twc: Difference between revisions
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[[Image:1twc.gif|left|200px]] | [[Image:1twc.gif|left|200px]] | ||
'''RNA polymerase II complexed with GTP''' | {{Structure | ||
|PDB= 1twc |SIZE=350|CAPTION= <scene name='initialview01'>1twc</scene>, resolution 3.0Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=GTP:GUANOSINE-5'-TRIPHOSPHATE'>GTP</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | |||
|GENE= | |||
}} | |||
'''RNA polymerase II complexed with GTP''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1TWC is a [ | 1TWC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWC OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center., Westover KD, Bushnell DA, Kornberg RD, Cell. 2004 Nov 12;119(4):481-9. PMID:[http:// | Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center., Westover KD, Bushnell DA, Kornberg RD, Cell. 2004 Nov 12;119(4):481-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15537538 15537538] | ||
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: multiprotein complex]] | [[Category: multiprotein complex]] | ||
[[Category: transcription]] | [[Category: transcription]] | ||
[[Category: zinc | [[Category: zinc motif]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:24:06 2008'' | ||
Revision as of 15:24, 20 March 2008
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| , resolution 3.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RNA polymerase II complexed with GTP
OverviewOverview
Binding of a ribonucleoside triphosphate to an RNA polymerase II transcribing complex, with base pairing to the template DNA, was revealed by X-ray crystallography. Binding of a mismatched nucleoside triphosphate was also detected, but in an adjacent site, inverted with respect to the correctly paired nucleotide. The results are consistent with a two-step mechanism of nucleotide selection, with initial binding to an entry (E) site beneath the active center in an inverted orientation, followed by rotation into the nucleotide addition (A) site for pairing with the template DNA. This mechanism is unrelated to that of single subunit RNA polymerases and so defines a new paradigm for the large, multisubunit enzymes. Additional findings from these studies include a third nucleotide binding site that may define the length of backtracked RNA; DNA double helix unwinding in advance of the polymerase active center; and extension of the diffraction limit of RNA polymerase II crystals to 2.3 A.
About this StructureAbout this Structure
1TWC is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center., Westover KD, Bushnell DA, Kornberg RD, Cell. 2004 Nov 12;119(4):481-9. PMID:15537538
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