1ts3: Difference between revisions
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'''H135A MUTANT OF TOXIC SHOCK SYNDROME TOXIN-1 FROM S. AUREUS''' | {{Structure | ||
|PDB= 1ts3 |SIZE=350|CAPTION= <scene name='initialview01'>1ts3</scene>, resolution 2.0Å | |||
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'''H135A MUTANT OF TOXIC SHOCK SYNDROME TOXIN-1 FROM S. AUREUS''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1TS3 is a [ | 1TS3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TS3 OCA]. | ||
==Reference== | ==Reference== | ||
Structures of five mutants of toxic shock syndrome toxin-1 with reduced biological activity., Earhart CA, Mitchell DT, Murray DL, Pinheiro DM, Matsumura M, Schlievert PM, Ohlendorf DH, Biochemistry. 1998 May 19;37(20):7194-202. PMID:[http:// | Structures of five mutants of toxic shock syndrome toxin-1 with reduced biological activity., Earhart CA, Mitchell DT, Murray DL, Pinheiro DM, Matsumura M, Schlievert PM, Ohlendorf DH, Biochemistry. 1998 May 19;37(20):7194-202. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9585531 9585531] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
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[[Category: toxin]] | [[Category: toxin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:22:24 2008'' | ||
Revision as of 15:22, 20 March 2008
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H135A MUTANT OF TOXIC SHOCK SYNDROME TOXIN-1 FROM S. AUREUS
OverviewOverview
The three-dimensional structures of five mutants of toxic shock syndrome toxin-1 (TSST-1) have been determined. These mutations are in the long central alpha helix and are useful in mapping portions of TSST-1 involved in superantigenicity and lethality. The T128A, H135A, Q139K, and I140T mutations appear to reduce superantigenicity by altering the properties of the T-cell receptor interaction surface. The Q136A mutation is at a largely buried site and causes a dramatic change in the conformation of the beta7-beta9 loop which covers the back of the central alpha helix. As this mutation has the unique ability to reduce the toxin's lethality in rabbits while retaining its superantigenicity, it raises the possibility that this rear loop mediates the ability of TSST-1 to induce lethality and suggests a route for producing nonlethal toxins for therapeutic development.
About this StructureAbout this Structure
1TS3 is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
ReferenceReference
Structures of five mutants of toxic shock syndrome toxin-1 with reduced biological activity., Earhart CA, Mitchell DT, Murray DL, Pinheiro DM, Matsumura M, Schlievert PM, Ohlendorf DH, Biochemistry. 1998 May 19;37(20):7194-202. PMID:9585531
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