1tp8: Difference between revisions
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[[Image:1tp8.gif|left|200px]] | [[Image:1tp8.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF A GALACTOSE SPECIFIC LECTIN FROM ARTOCARPUS HIRSUTA IN COMPLEX WITH METHYL-a-D-GALACTOSE''' | {{Structure | ||
|PDB= 1tp8 |SIZE=350|CAPTION= <scene name='initialview01'>1tp8</scene>, resolution 3.0Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=AMG:ALPHA-METHYL-D-GALACTOSIDE'>AMG</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF A GALACTOSE SPECIFIC LECTIN FROM ARTOCARPUS HIRSUTA IN COMPLEX WITH METHYL-a-D-GALACTOSE''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1TP8 is a [ | 1TP8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Artocarpus_hirsutus Artocarpus hirsutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TP8 OCA]. | ||
==Reference== | ==Reference== | ||
Two orthorhombic crystal structures of a galactose-specific lectin from Artocarpus hirsuta in complex with methyl-alpha-D-galactose., Rao KN, Suresh CG, Katre UV, Gaikwad SM, Khan MI, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1404-12. Epub 2004, Jul 21. PMID:[http:// | Two orthorhombic crystal structures of a galactose-specific lectin from Artocarpus hirsuta in complex with methyl-alpha-D-galactose., Rao KN, Suresh CG, Katre UV, Gaikwad SM, Khan MI, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1404-12. Epub 2004, Jul 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15272163 15272163] | ||
[[Category: Artocarpus hirsutus]] | [[Category: Artocarpus hirsutus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: jacalin family]] | [[Category: jacalin family]] | ||
[[Category: methyl--d-galactose]] | [[Category: methyl--d-galactose]] | ||
[[Category: moraceae plant | [[Category: moraceae plant lectin]] | ||
[[Category: post-translational modification]] | [[Category: post-translational modification]] | ||
[[Category: three-dimensional structure]] | [[Category: three-dimensional structure]] | ||
[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:21:21 2008'' | ||
Revision as of 15:21, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A GALACTOSE SPECIFIC LECTIN FROM ARTOCARPUS HIRSUTA IN COMPLEX WITH METHYL-a-D-GALACTOSE
OverviewOverview
Based on their carbohydrate specificity, the jacalin family of lectins can be divided into two groups: galactose-specific and mannose-specific. The former are cytoplasmic proteins, whereas the latter are localized in the storage vacuoles of cells. It has been proposed that the post-translational modification in some of the lectins that splits their polypeptide chains into two may be crucial for galactose specificity. The mannose-specific members of the family are single-chain proteins that lack the above modification. Although the galactose-specific and the mannose-specific jacalin-type lectins differ in their sequences, they share a common fold: the beta-prism I fold, which is characteristic of Moraceae plant lectins. Here, two crystal structures of a jacalin-related lectin from Artocarpus hirsuta, which is specific for galactose, in complex with methyl-alpha-D-galactose are reported. The lectin crystallized in two orthorhombic forms and one hexagonal form under similar conditions. The crystals had an unusually high solvent content. The structure was solved using the molecular-replacement method using the jacalin structure as a search model. The two orthorhombic forms were refined using data to 2.5 and 3.0 A resolution, respectively. The structures of the A. hirsuta lectin and jacalin are identical. In orthorhombic form I the crystal packing provides three different micro-environments for sugar binding in the same crystal. The observed difference in the specificity for oligosaccharides between the A. hirsuta lectin and jacalin could only be explained based on differences in the molecular associations in the packing and variation of the C-terminal length of the beta-chain. The observed insecticidal activity of A. hirsuta lectin may arise from its similar fold to domain II of the unrelated delta-endotoxin from Bacillus thuringiensis.
About this StructureAbout this Structure
1TP8 is a Protein complex structure of sequences from Artocarpus hirsutus. Full crystallographic information is available from OCA.
ReferenceReference
Two orthorhombic crystal structures of a galactose-specific lectin from Artocarpus hirsuta in complex with methyl-alpha-D-galactose., Rao KN, Suresh CG, Katre UV, Gaikwad SM, Khan MI, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1404-12. Epub 2004, Jul 21. PMID:15272163
Page seeded by OCA on Thu Mar 20 14:21:21 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Artocarpus hirsutus
- Protein complex
- Gaikwad, S M.
- Katre, U V.
- Khan, M I.
- Rao, K N.
- Suresh, C G.
- AMG
- -prism i fold
- Artocarpus hirsuta
- Carbohydrate specificity
- Jacalin family
- Methyl--d-galactose
- Moraceae plant lectin
- Post-translational modification
- Three-dimensional structure
- X-ray crystallography