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{{STRUCTURE_2m1w| PDB=2m1w | SCENE= }}
==TICAM-2 TIR domain==
===TICAM-2 TIR domain===
<StructureSection load='2m1w' size='340' side='right' caption='[[2m1w]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
{{ABSTRACT_PUBMED_24255114}}
== Structural highlights ==
<table><tr><td colspan='2'>[[2m1w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M1W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M1W FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2m1x|2m1x]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TICAM2, TIRAP3, TIRP, TRAM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m1w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2m1w RCSB], [http://www.ebi.ac.uk/pdbsum/2m1w PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Homotypic and heterotypic interactions between Toll/interleukin-1 receptor (TIR) domains in Toll-like receptors (TLRs) and downstream adaptors are essential to evoke innate immune responses. However, such oligomerization properties present intrinsic difficulties in structural studies of TIR domains. Here, using BB-loop mutations that disrupt homotypic interactions, we determined the structures of the monomeric TIR domain-containing adaptor molecule (TICAM)-1 and TICAM-2 TIR domains. Docking of the monomeric structures, together with yeast two hybrid-based mutagenesis assays, reveals that the homotypic interaction between TICAM-2 TIR is indispensable to present a scaffold for recruiting the monomeric moiety of the TICAM-1 TIR dimer. This result proposes a unique idea that oligomerization of upstream TIR domains is crucial for binding of downstream TIR domains. Furthermore, the bivalent nature of each TIR domain dimer can generate a large signaling complex under the activated TLRs, which would recruit downstream signaling molecules efficiently. This model is consistent with previous reports that BB-loop mutants completely abrogate downstream signaling.


==Function==
Structures and interface mapping of the TIR domain-containing adaptor molecules involved in interferon signaling.,Enokizono Y, Kumeta H, Funami K, Horiuchi M, Sarmiento J, Yamashita K, Standley DM, Matsumoto M, Seya T, Inagaki F Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):19908-13. doi:, 10.1073/pnas.1222811110. Epub 2013 Nov 19. PMID:24255114<ref>PMID:24255114</ref>
[[http://www.uniprot.org/uniprot/TCAM2_HUMAN TCAM2_HUMAN]] Functions as sorting adapter in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1; signaling is proposed to occur in early endosomes after endocytosis of TLR4. May also be involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB; however, reports are controversial. Involved in IL-18 signaling and is proposed to function as a sorting adaptor for MYD88 in IL-18 signaling during adaptive immune response.<ref>PMID:12721283</ref> <ref>PMID:14519765</ref> <ref>PMID:14517278</ref> <ref>PMID:194121844</ref> <ref>PMID:16603631</ref> <ref>PMID:16757566</ref> <ref>PMID:22685567</ref>  Isoform 2: Proposed to inhibit LPS-TLR4 signaling at the late endosome by interaction with isoform 1 thereby disrupting the association of isoform 1 with TICAM1. May be involved in TLR4 degradation in late endosomes.<ref>PMID:12721283</ref> <ref>PMID:14519765</ref> <ref>PMID:14517278</ref> <ref>PMID:194121844</ref> <ref>PMID:16603631</ref> <ref>PMID:16757566</ref> <ref>PMID:22685567</ref>


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[2m1w]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M1W OCA].
</div>
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:024255114</ref><references group="xtra"/><references/>
__TOC__
[[Category: Enokizono, Y.]]
</StructureSection>
[[Category: Funami, K.]]
[[Category: Human]]
[[Category: Horiuchi, M.]]
[[Category: Enokizono, Y]]
[[Category: Inagaki, F.]]
[[Category: Funami, K]]
[[Category: Kumeta, H.]]
[[Category: Horiuchi, M]]
[[Category: Matsumoto, M.]]
[[Category: Inagaki, F]]
[[Category: Sarmiento, J.]]
[[Category: Kumeta, H]]
[[Category: Seya, T.]]
[[Category: Matsumoto, M]]
[[Category: Standley, D M.]]
[[Category: Sarmiento, J]]
[[Category: Yamashita, K.]]
[[Category: Seya, T]]
[[Category: Standley, D M]]
[[Category: Yamashita, K]]
[[Category: Immune system]]
[[Category: Immune system]]
[[Category: Innate immunity]]
[[Category: Innate immunity]]

Revision as of 09:22, 22 December 2014

TICAM-2 TIR domainTICAM-2 TIR domain

Structural highlights

2m1w is a 1 chain structure with sequence from Human. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:TICAM2, TIRAP3, TIRP, TRAM (HUMAN)
Resources:FirstGlance, OCA, RCSB, PDBsum

Publication Abstract from PubMed

Homotypic and heterotypic interactions between Toll/interleukin-1 receptor (TIR) domains in Toll-like receptors (TLRs) and downstream adaptors are essential to evoke innate immune responses. However, such oligomerization properties present intrinsic difficulties in structural studies of TIR domains. Here, using BB-loop mutations that disrupt homotypic interactions, we determined the structures of the monomeric TIR domain-containing adaptor molecule (TICAM)-1 and TICAM-2 TIR domains. Docking of the monomeric structures, together with yeast two hybrid-based mutagenesis assays, reveals that the homotypic interaction between TICAM-2 TIR is indispensable to present a scaffold for recruiting the monomeric moiety of the TICAM-1 TIR dimer. This result proposes a unique idea that oligomerization of upstream TIR domains is crucial for binding of downstream TIR domains. Furthermore, the bivalent nature of each TIR domain dimer can generate a large signaling complex under the activated TLRs, which would recruit downstream signaling molecules efficiently. This model is consistent with previous reports that BB-loop mutants completely abrogate downstream signaling.

Structures and interface mapping of the TIR domain-containing adaptor molecules involved in interferon signaling.,Enokizono Y, Kumeta H, Funami K, Horiuchi M, Sarmiento J, Yamashita K, Standley DM, Matsumoto M, Seya T, Inagaki F Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):19908-13. doi:, 10.1073/pnas.1222811110. Epub 2013 Nov 19. PMID:24255114[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Enokizono Y, Kumeta H, Funami K, Horiuchi M, Sarmiento J, Yamashita K, Standley DM, Matsumoto M, Seya T, Inagaki F. Structures and interface mapping of the TIR domain-containing adaptor molecules involved in interferon signaling. Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):19908-13. doi:, 10.1073/pnas.1222811110. Epub 2013 Nov 19. PMID:24255114 doi:http://dx.doi.org/10.1073/pnas.1222811110
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