1hz2: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1hz2]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HZ2 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1hz2]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HZ2 FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TME:PROPANE'>TME</scene>< | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TME:PROPANE'>TME</scene></td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hz2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hz2 RCSB], [http://www.ebi.ac.uk/pdbsum/1hz2 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hz2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hz2 RCSB], [http://www.ebi.ac.uk/pdbsum/1hz2 PDBsum]</span></td></tr> | ||
<table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Dooley, P A | [[Category: Dooley, P A]] | ||
[[Category: Harris, C M | [[Category: Harris, C M]] | ||
[[Category: Harris, T M | [[Category: Harris, T M]] | ||
[[Category: Korbel, G A | [[Category: Korbel, G A]] | ||
[[Category: Nechev, L V | [[Category: Nechev, L V]] | ||
[[Category: Shearer, J | [[Category: Shearer, J]] | ||
[[Category: Stone, M P | [[Category: Stone, M P]] | ||
[[Category: Tsarouhtsis, D | [[Category: Tsarouhtsis, D]] | ||
[[Category: Zegar, I S | [[Category: Zegar, I S]] | ||
[[Category: Dna]] | [[Category: Dna]] | ||
[[Category: Dna duplex]] | [[Category: Dna duplex]] | ||
[[Category: Interstrand crosslink]] | [[Category: Interstrand crosslink]] | ||
Revision as of 08:44, 22 December 2014
SOLUTION NMR STRUCTURE OF SELF-COMPLEMENTARY DUPLEX 5'-D(AGGCG*CCT)2 CONTAINING A TRIMETHYLENE CROSSLINK AT THE N2 POSITION OF G*. MODEL OF A MALONDIALDEHYDE CROSSLINKSOLUTION NMR STRUCTURE OF SELF-COMPLEMENTARY DUPLEX 5'-D(AGGCG*CCT)2 CONTAINING A TRIMETHYLENE CROSSLINK AT THE N2 POSITION OF G*. MODEL OF A MALONDIALDEHYDE CROSSLINK
Structural highlights
Publication Abstract from PubMedMalondialdehyde (MDA), a known mutagen and suspected carcinogen, is a product of lipid peroxidation and byproduct of eicosanoid biosynthesis. MDA can react with DNA to generate potentially mutagenic adducts on adenine, cytosine, and particularly guanine. In addition, repair-dependent frame shift mutations in a GCGCGC region of Salmonella typhimurium hisD3052 have been attributed to formation of interstrand cross-links (Mukai, F. H. and Goldstein, B. D. Science 1976, 191, 868--869). The cross-linked species is unstable and has never been characterized but has been postulated to be a bis-imino linkage between N(2) positions of guanines. An analogous linkage has now been investigated as a stable surrogate using the self-complementary oligodeoxynucleotide sequence 5'-d(AGGCG*CCT)(2,) in which G* represents guanines linked via a trimethylene chain between N(2) positions. The solution structure, obtained by NMR spectroscopy and molecular dynamics using a simulated annealing protocol, revealed the cross-link only minimally distorts duplex structure in the region of the cross-link. The tether is accommodated by partially unwinding the duplex at the lesion site to produce a bulge and tipping the guanine residues; the two guanines and the tether attain a nearly planar conformation. This distortion did not result in significant bending of the DNA, a result which was confirmed by gel electrophoresis studies of multimers of a 21-mer duplex containing the cross-link. Structural studies of an oligodeoxynucleotide containing a trimethylene interstrand cross-link in a 5'-(CpG) motif: model of a malondialdehyde cross-link.,Dooley PA, Tsarouhtsis D, Korbel GA, Nechev LV, Shearer J, Zegar IS, Harris CM, Stone MP, Harris TM J Am Chem Soc. 2001 Feb 28;123(8):1730-9. PMID:11456774[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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