1t3a: Difference between revisions
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[[Image:1t3a.jpg|left|200px]] | [[Image:1t3a.jpg|left|200px]] | ||
'''Crystal structure of Clostridium botulinum neurotoxin type E catalytic domain''' | {{Structure | ||
|PDB= 1t3a |SIZE=350|CAPTION= <scene name='initialview01'>1t3a</scene>, resolution 2.16Å | |||
|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site'>AC1</scene> and <scene name='pdbsite=AC2:Zn+Binding+Site'>AC2</scene> | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] | |||
|GENE= | |||
}} | |||
'''Crystal structure of Clostridium botulinum neurotoxin type E catalytic domain''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1T3A is a [ | 1T3A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T3A OCA]. | ||
==Reference== | ==Reference== | ||
Structural analysis of botulinum neurotoxin type E catalytic domain and its mutant Glu212-->Gln reveals the pivotal role of the Glu212 carboxylate in the catalytic pathway., Agarwal R, Eswaramoorthy S, Kumaran D, Binz T, Swaminathan S, Biochemistry. 2004 Jun 1;43(21):6637-44. PMID:[http:// | Structural analysis of botulinum neurotoxin type E catalytic domain and its mutant Glu212-->Gln reveals the pivotal role of the Glu212 carboxylate in the catalytic pathway., Agarwal R, Eswaramoorthy S, Kumaran D, Binz T, Swaminathan S, Biochemistry. 2004 Jun 1;43(21):6637-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15157097 15157097] | ||
[[Category: Bontoxilysin]] | [[Category: Bontoxilysin]] | ||
[[Category: Clostridium botulinum]] | [[Category: Clostridium botulinum]] | ||
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[[Category: light chain]] | [[Category: light chain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:13:10 2008'' | ||
Revision as of 15:13, 20 March 2008
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| , resolution 2.16Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | and | ||||||
| Ligands: | and | ||||||
| Activity: | Bontoxilysin, with EC number 3.4.24.69 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Clostridium botulinum neurotoxin type E catalytic domain
OverviewOverview
The seven serotypes of botulinum neurotoxins (A-G) produced by Clostridium botulinum share significant sequence homology and structural similarity. The functions of their individual domains and the modes of action are also similar. However, the substrate specificity and the peptide bond cleavage selectivity of their catalytic domains are different. The reason for this unique specificity of botulinum neurotoxins is still baffling. If an inhibitor leading to a therapeutic drug common to all serotypes is to be developed, it is essential to understand the differences in their three-dimensional structures that empower them with this unique characteristic. Accordingly, high-resolution structures of all serotypes are required, and toward achieving this goal the crystal structure of the catalytic domain of C. botulinum neurotoxin type E has been determined to 2.1 A resolution. The crystal structure of the inactive mutant Glu212-->Gln of this protein has also been determined. While the overall conformation is unaltered in the active site, the position of the nucleophilic water changes in the mutant, thereby causing it to lose its ability to activate the catalytic reaction. The structure explains the importance of the nucleophilic water and the charge on Glu212. The structural differences responsible for the loss of activity of the mutant provide a common model for the catalytic pathway of Clostridium neurotoxins since Glu212 is conserved and has a similar role in all serotypes. This or a more nonconservative mutant (e.g., Glu212-->Ala) could provide a novel, genetically modified protein vaccine for botulinum.
About this StructureAbout this Structure
1T3A is a Single protein structure of sequence from Clostridium botulinum. Full crystallographic information is available from OCA.
ReferenceReference
Structural analysis of botulinum neurotoxin type E catalytic domain and its mutant Glu212-->Gln reveals the pivotal role of the Glu212 carboxylate in the catalytic pathway., Agarwal R, Eswaramoorthy S, Kumaran D, Binz T, Swaminathan S, Biochemistry. 2004 Jun 1;43(21):6637-44. PMID:15157097
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