4l13: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal structure of Ligand Free EGFP-based Calcium Sensor CatchER== | |||
<StructureSection load='4l13' size='340' side='right' caption='[[4l13]], [[Resolution|resolution]] 1.66Å' scene=''> | |||
{ | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4l13]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L13 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L13 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4l12|4l12]], [[4l1i|4l1i]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l13 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l13 RCSB], [http://www.ebi.ac.uk/pdbsum/4l13 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Calcium ions, which are important signaling molecules, can be detected in the endoplasmic reticulum by an engineered mutant of green fluorescent protein (GFP) designated CatchER with a fast off-rate. High resolution (1.78-1.20 A) crystal structures were analyzed for CatchER in the apo form and in complexes with calcium or gadolinium to probe the binding site for metal ions. While CatchER exhibits a 1:1 binding stoichiometry in solution, two positions were observed for each of the metal ions bound within the hand-like site formed by the carboxylate side chains of the mutated residues S147E, S202D, Q204E, F223E and T225E that may be responsible for its fast kinetic properties. Comparison of the structures of CatchER, wild-type GFP and enhanced GFP confirmed that different conformations of Thr203 and Glu222 are associated with the two forms of Tyr66 of the chromophore which are responsible for the absorbance wavelengths of the different proteins. Calcium binding to CatchER may shift the equilibrium for conformational population of the Glu222 side chain and lead to further changes in its optical properties. | |||
Structural basis for a hand-like site in the calcium sensor CatchER with fast kinetics.,Zhang Y, Reddish F, Tang S, Zhuo Y, Wang YF, Yang JJ, Weber IT Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2309-19. doi:, 10.1107/S0907444913021306. Epub 2013 Nov 19. PMID:24311573<ref>PMID:24311573</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
== | <references/> | ||
__TOC__ | |||
[[Category: Weber, I T | </StructureSection> | ||
[[Category: Zhang, Y | [[Category: Aeqvi]] | ||
[[Category: Weber, I T]] | |||
[[Category: Zhang, Y]] | |||
[[Category: Calcium binding protein]] | [[Category: Calcium binding protein]] | ||
[[Category: Calcium sensor]] | [[Category: Calcium sensor]] | ||
Revision as of 21:52, 21 December 2014
Crystal structure of Ligand Free EGFP-based Calcium Sensor CatchERCrystal structure of Ligand Free EGFP-based Calcium Sensor CatchER
Structural highlights
Publication Abstract from PubMedCalcium ions, which are important signaling molecules, can be detected in the endoplasmic reticulum by an engineered mutant of green fluorescent protein (GFP) designated CatchER with a fast off-rate. High resolution (1.78-1.20 A) crystal structures were analyzed for CatchER in the apo form and in complexes with calcium or gadolinium to probe the binding site for metal ions. While CatchER exhibits a 1:1 binding stoichiometry in solution, two positions were observed for each of the metal ions bound within the hand-like site formed by the carboxylate side chains of the mutated residues S147E, S202D, Q204E, F223E and T225E that may be responsible for its fast kinetic properties. Comparison of the structures of CatchER, wild-type GFP and enhanced GFP confirmed that different conformations of Thr203 and Glu222 are associated with the two forms of Tyr66 of the chromophore which are responsible for the absorbance wavelengths of the different proteins. Calcium binding to CatchER may shift the equilibrium for conformational population of the Glu222 side chain and lead to further changes in its optical properties. Structural basis for a hand-like site in the calcium sensor CatchER with fast kinetics.,Zhang Y, Reddish F, Tang S, Zhuo Y, Wang YF, Yang JJ, Weber IT Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2309-19. doi:, 10.1107/S0907444913021306. Epub 2013 Nov 19. PMID:24311573[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||||||