1t1d: Difference between revisions

Revision as of 15:12, 20 March 2008

, resolution 1.51Å

Coordinates:

CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL

OverviewOverview

The N-terminal, cytoplasmic tetramerization domain (T1) of voltage-gated K+ channels encodes molecular determinants for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. Crystal structures of T1 tetramers from Shaw and Shaker subfamilies reveal a common four-layered scaffolding. Within layer 4, on the hypothetical membrane-facing side of the tetramer, the Shaw T1 tetramer contains four zinc ions; each is coordinated by a histidine and two cysteines from one monomer and by one cysteine from an adjacent monomer. The amino acids involved in coordinating the Zn2+ ion occur in a HX5CX20CC sequence motif that is highly conserved among all Shab, Shaw and Shal subfamily members, but is not found in Shaker subfamily members. We demonstrate by coimmunoprecipitation that a few characteristic residues in the subunit interface are crucial for subfamily-specific tetramerization of the T1 domains.

About this StructureAbout this Structure

1T1D is a Single protein structure of sequence from Aplysia californica. Full crystallographic information is available from OCA.

ReferenceReference

Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels., Bixby KA, Nanao MH, Shen NV, Kreusch A, Bellamy H, Pfaffinger PJ, Choe S, Nat Struct Biol. 1999 Jan;6(1):38-43. PMID:9886290

Page seeded by OCA on Thu Mar 20 14:12:26 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1t1d.gif|left|200px]]<br /><applet load="1t1d" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1t1d.gif|left|200px]]
-caption="1t1d, resolution 1.51&Aring;" />
-'''CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL'''<br />
+ {{Structure
+|PDB= 1t1d |SIZE=350|CAPTION= <scene name='initialview01'>1t1d</scene>, resolution 1.51&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-T1D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1D OCA].
+T1D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1D OCA].
 ==Reference==
-Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels., Bixby KA, Nanao MH, Shen NV, Kreusch A, Bellamy H, Pfaffinger PJ, Choe S, Nat Struct Biol. 1999 Jan;6(1):38-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9886290 9886290]
+Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels., Bixby KA, Nanao MH, Shen NV, Kreusch A, Bellamy H, Pfaffinger PJ, Choe S, Nat Struct Biol. 1999 Jan;6(1):38-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9886290 9886290]
 [[Category: Aplysia californica]]
 [[Category: Single protein]]
@@ Line 18: / Line 27: @@
 [[Category: Stevens, C F.]]
 [[Category: aplysia kv1 1]]
-[[Category: potassium channels]]
+[[Category: potassium channel]]
 [[Category: proton transport]]
 [[Category: tetramerization domain]]
 [[Category: x-ray structure]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:08:53 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:12:26 2008''